Dexamethasone and nitric oxide synthase gene expression in brain

Eiji Suzuki, Futoshi Shintani, Toshio Nakaki, Kiyoshi Nagata, Yasushi Yamazoe, Nobuchika Fujita, Masahiro Asai, Shigenobu Kanba

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Abstract

Systemic administration of lipopolysaccharide (LPS), which causes endotoxemia and systemic inflammation, has been reported to induce expression of the gene for type II inducible nitric oxide synthase (iNOS) in peripheral organs. This study was carried out to examine whether intraperitoneally injected LPS elicits the expression of iNOS messenger ribonucleic acid (mRNA) in the rat brain. We also investigated whether intraperitoneal treatment with dexamethasone (DEX) prevents this induction. To determine levels of iNOS mRNA, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method was employed. Treatment with LPS induced the expression of iNOS mRNA in various brain regions, accounting for approximately 1 x 105 to 4 x 105 molecules per μg of poly A+ RNA, and these inductions were markedly suppressed by DEX. The results suggest that, during systemic inflammation, iNOS mRNA induction occurs in brain through a DEX-sensitive mechanism.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalJournal of Psychiatry and Neuroscience
Volume22
Issue number2
Publication statusPublished - May 28 1997

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All Science Journal Classification (ASJC) codes

  • Psychiatry and Mental health
  • Biological Psychiatry
  • Pharmacology (medical)

Cite this

Suzuki, E., Shintani, F., Nakaki, T., Nagata, K., Yamazoe, Y., Fujita, N., ... Kanba, S. (1997). Dexamethasone and nitric oxide synthase gene expression in brain. Journal of Psychiatry and Neuroscience, 22(2), 105-110.