Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro

Naoto Burioka, Miyako Takata, Yoko Okano, Shigehiro Ohdo, Yasushi Fukuoka, Masanori Miyata, Hiroshi Takane, Masahiro Endo, Hisashi Suyama, Eiji Shimizu

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34 Citations (Scopus)

Abstract

We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.

Original languageEnglish
Pages (from-to)585-590
Number of pages6
JournalChronobiology International
Volume22
Issue number3
DOIs
Publication statusPublished - Aug 24 2005

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

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    Burioka, N., Takata, M., Okano, Y., Ohdo, S., Fukuoka, Y., Miyata, M., Takane, H., Endo, M., Suyama, H., & Shimizu, E. (2005). Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro. Chronobiology International, 22(3), 585-590. https://doi.org/10.1081/CBI-200062416