Dexamethasone stimulates osteoclast-like cell formation by inhibiting granulocyte-macrophage colony-stimulating factor production in mouse bone marrow cultures

Toshihide Shuto, Toshio Kukita, Masato Hirata, Eijiro Jimi, Toshitaka Koga

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We investigated the effect of glucocorticoid on the generation of osteoclasts. In mouse bone marrow culture systems, dexamethasone, a synthetic glucocorticoid analog, enhanced osteoclast-like cell formation induced by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in a dose-dependent manner. Conversely, dexamethasone inhibited the endogenous production of granulocyte macrophage colony-stimulating factor (GM-CSF) in bone marrow cultures. GM- CSF, when added exogenously, suppressed not only the osteoclast-like cell formation induced by 1,25-(OH)2D3, but also the stimulatory effect of dexamethasone, and addition of anti-GM-CSF neutralizing antibody to the cultures significantly increased the osteoclast-like cell formation induced by 1,25-(OH)2D3. These observations suggest that dexamethasone directly affects bone marrow cells and enhances osteoclast generation by inhibiting the endogenous production of GM-CSF, which may function as a negative regulator of osteoclast formation.

Original languageEnglish
Pages (from-to)1121-1126
Number of pages6
JournalEndocrinology
Volume134
Issue number3
DOIs
Publication statusPublished - Mar 1994

All Science Journal Classification (ASJC) codes

  • Endocrinology

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