Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species

Noriaki Kamio, Sumio Akifusa, Yoshihisa Yamashita

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub-cellular signalling pathways, had a role on gAd-induced ROS generation in RAW 264 cells. Administration of R59022, a specific inhibitor for DGK, reduced gAd-induced ROS generation and NO release. RAW 264 cell expressed DGKα mRNA. Depression of DGKα mRNA by RNA interference significantly reduced the ROS generation in response to gAd treatment. Interestingly, transfection with the DGKα-specific small interfering RNA attenuated the expression level of Nox1 mRNA in gAd-treated RAW 264 cells. In addition, the DGKα knockdown with siRNA suppressed gAd-induced NO release.

Original languageEnglish
Pages (from-to)336-341
Number of pages6
JournalFree Radical Research
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry

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