Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene.

Toshinori Kawanami, Kazuhiro Yatera, Kazumasa Fukuda, Kei Yamasaki, Masamizu Kunimoto, Shuya Nagata, Chinatsu Nishida, Hiroshi Ishimoto, Midori Ogawa, Hatsumi Taniguchi, Hiroshi Mukae

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Pneumonia is the fourth leading cause of death in Japan. Accurate and rapid detection of the causative pathogen(s) is necessary and important for appropriate antimicrobial treatment, especially in patients with rapidly progressive pneumonia or immunocompromised patients. Conventional methods, such as cultivations, detection of urinary antigens or PCR amplification of specific genes, inevitably require the precise presumption of potential pathogens in each case, and pneumonia caused by unanticipated microorganisms might lead to inadequate antimicrobial treatments and unfortunate consequences. We herein report an immunocompromised female patient (69 years old) with fulminant pneumonia caused by Legionella (L.) pneumophila serogroup 8. Ordinary cultivation methods and urinary antigen detection failed to identify the causative organisms. Accordingly, DNA was extracted from the bronchoalveolar lavage fluid and used for the PCR-based cloning of the bacterial 16S rRNA gene. Sequencing analysis of the isolated clones revealed the predominance of L. pneumophila. Based on this information, the patient received an appropriate and successful antimicrobial treatment. In addition, L. pneumophila serogroup 8 was identified with culturing the bronchoalveolar lavage fluid and serotyping with L. pneumophila antisera. The 16S rRNA gene sequencing analysis can reveal the potential pathogens without any presumption about the organism, and can evaluate the kinds and ratio of bacterial species in each specimen. In conclusion, this cultivation-independent method is a potential diagnostic modality for pneumonia, especially in patients with rapidly progressive pneumonia or those who are immunocompromised.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalThe Tohoku journal of experimental medicine
Volume225
Issue number1
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Fingerprint

Legionella pneumophila
Pathogens
rRNA Genes
Sequence Analysis
Pneumonia
Genes
Antigens
Fluids
Cloning
Bronchoalveolar Lavage Fluid
Immunocompromised Host
Microorganisms
Amplification
Immune Sera
Serotyping
Polymerase Chain Reaction
Gene Amplification
DNA
Serogroup
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene. / Kawanami, Toshinori; Yatera, Kazuhiro; Fukuda, Kazumasa; Yamasaki, Kei; Kunimoto, Masamizu; Nagata, Shuya; Nishida, Chinatsu; Ishimoto, Hiroshi; Ogawa, Midori; Taniguchi, Hatsumi; Mukae, Hiroshi.

In: The Tohoku journal of experimental medicine, Vol. 225, No. 1, 01.01.2011, p. 65-69.

Research output: Contribution to journalArticle

Kawanami, T, Yatera, K, Fukuda, K, Yamasaki, K, Kunimoto, M, Nagata, S, Nishida, C, Ishimoto, H, Ogawa, M, Taniguchi, H & Mukae, H 2011, 'Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene.', The Tohoku journal of experimental medicine, vol. 225, no. 1, pp. 65-69. https://doi.org/10.1620/tjem.225.65
Kawanami, Toshinori ; Yatera, Kazuhiro ; Fukuda, Kazumasa ; Yamasaki, Kei ; Kunimoto, Masamizu ; Nagata, Shuya ; Nishida, Chinatsu ; Ishimoto, Hiroshi ; Ogawa, Midori ; Taniguchi, Hatsumi ; Mukae, Hiroshi. / Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene. In: The Tohoku journal of experimental medicine. 2011 ; Vol. 225, No. 1. pp. 65-69.
@article{52b701e83267401881f2f396691e89de,
title = "Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene.",
abstract = "Pneumonia is the fourth leading cause of death in Japan. Accurate and rapid detection of the causative pathogen(s) is necessary and important for appropriate antimicrobial treatment, especially in patients with rapidly progressive pneumonia or immunocompromised patients. Conventional methods, such as cultivations, detection of urinary antigens or PCR amplification of specific genes, inevitably require the precise presumption of potential pathogens in each case, and pneumonia caused by unanticipated microorganisms might lead to inadequate antimicrobial treatments and unfortunate consequences. We herein report an immunocompromised female patient (69 years old) with fulminant pneumonia caused by Legionella (L.) pneumophila serogroup 8. Ordinary cultivation methods and urinary antigen detection failed to identify the causative organisms. Accordingly, DNA was extracted from the bronchoalveolar lavage fluid and used for the PCR-based cloning of the bacterial 16S rRNA gene. Sequencing analysis of the isolated clones revealed the predominance of L. pneumophila. Based on this information, the patient received an appropriate and successful antimicrobial treatment. In addition, L. pneumophila serogroup 8 was identified with culturing the bronchoalveolar lavage fluid and serotyping with L. pneumophila antisera. The 16S rRNA gene sequencing analysis can reveal the potential pathogens without any presumption about the organism, and can evaluate the kinds and ratio of bacterial species in each specimen. In conclusion, this cultivation-independent method is a potential diagnostic modality for pneumonia, especially in patients with rapidly progressive pneumonia or those who are immunocompromised.",
author = "Toshinori Kawanami and Kazuhiro Yatera and Kazumasa Fukuda and Kei Yamasaki and Masamizu Kunimoto and Shuya Nagata and Chinatsu Nishida and Hiroshi Ishimoto and Midori Ogawa and Hatsumi Taniguchi and Hiroshi Mukae",
year = "2011",
month = "1",
day = "1",
doi = "10.1620/tjem.225.65",
language = "English",
volume = "225",
pages = "65--69",
journal = "Tohoku Journal of Experimental Medicine",
issn = "0040-8727",
publisher = "Tohoku University Medical Press",
number = "1",

}

TY - JOUR

T1 - Diagnosis of fulminant pneumonia caused by Legionella pneumophila serogroup 8 with the sequence analysis of the 16S rRNA gene.

AU - Kawanami, Toshinori

AU - Yatera, Kazuhiro

AU - Fukuda, Kazumasa

AU - Yamasaki, Kei

AU - Kunimoto, Masamizu

AU - Nagata, Shuya

AU - Nishida, Chinatsu

AU - Ishimoto, Hiroshi

AU - Ogawa, Midori

AU - Taniguchi, Hatsumi

AU - Mukae, Hiroshi

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Pneumonia is the fourth leading cause of death in Japan. Accurate and rapid detection of the causative pathogen(s) is necessary and important for appropriate antimicrobial treatment, especially in patients with rapidly progressive pneumonia or immunocompromised patients. Conventional methods, such as cultivations, detection of urinary antigens or PCR amplification of specific genes, inevitably require the precise presumption of potential pathogens in each case, and pneumonia caused by unanticipated microorganisms might lead to inadequate antimicrobial treatments and unfortunate consequences. We herein report an immunocompromised female patient (69 years old) with fulminant pneumonia caused by Legionella (L.) pneumophila serogroup 8. Ordinary cultivation methods and urinary antigen detection failed to identify the causative organisms. Accordingly, DNA was extracted from the bronchoalveolar lavage fluid and used for the PCR-based cloning of the bacterial 16S rRNA gene. Sequencing analysis of the isolated clones revealed the predominance of L. pneumophila. Based on this information, the patient received an appropriate and successful antimicrobial treatment. In addition, L. pneumophila serogroup 8 was identified with culturing the bronchoalveolar lavage fluid and serotyping with L. pneumophila antisera. The 16S rRNA gene sequencing analysis can reveal the potential pathogens without any presumption about the organism, and can evaluate the kinds and ratio of bacterial species in each specimen. In conclusion, this cultivation-independent method is a potential diagnostic modality for pneumonia, especially in patients with rapidly progressive pneumonia or those who are immunocompromised.

AB - Pneumonia is the fourth leading cause of death in Japan. Accurate and rapid detection of the causative pathogen(s) is necessary and important for appropriate antimicrobial treatment, especially in patients with rapidly progressive pneumonia or immunocompromised patients. Conventional methods, such as cultivations, detection of urinary antigens or PCR amplification of specific genes, inevitably require the precise presumption of potential pathogens in each case, and pneumonia caused by unanticipated microorganisms might lead to inadequate antimicrobial treatments and unfortunate consequences. We herein report an immunocompromised female patient (69 years old) with fulminant pneumonia caused by Legionella (L.) pneumophila serogroup 8. Ordinary cultivation methods and urinary antigen detection failed to identify the causative organisms. Accordingly, DNA was extracted from the bronchoalveolar lavage fluid and used for the PCR-based cloning of the bacterial 16S rRNA gene. Sequencing analysis of the isolated clones revealed the predominance of L. pneumophila. Based on this information, the patient received an appropriate and successful antimicrobial treatment. In addition, L. pneumophila serogroup 8 was identified with culturing the bronchoalveolar lavage fluid and serotyping with L. pneumophila antisera. The 16S rRNA gene sequencing analysis can reveal the potential pathogens without any presumption about the organism, and can evaluate the kinds and ratio of bacterial species in each specimen. In conclusion, this cultivation-independent method is a potential diagnostic modality for pneumonia, especially in patients with rapidly progressive pneumonia or those who are immunocompromised.

UR - http://www.scopus.com/inward/record.url?scp=84555204277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84555204277&partnerID=8YFLogxK

U2 - 10.1620/tjem.225.65

DO - 10.1620/tjem.225.65

M3 - Article

VL - 225

SP - 65

EP - 69

JO - Tohoku Journal of Experimental Medicine

JF - Tohoku Journal of Experimental Medicine

SN - 0040-8727

IS - 1

ER -