Diazepam attenuates conditioned histamine release in guinea pigs

Masahiro Irie, Shoji Nagata, Yutaka Endo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To clarify the possibility of pharmacological mediation on classical conditioning-associated asthmatic response, the effect of diazepam on an odor-induced conditioned histamine release was investigated in ovalbumin (OA)-sensitized guinea pigs, i.e. a model of bronchial asthma. The animals received conditioning sessions in which an antigen (OA) as the unconditioned stimulus and an odor (dimethylsulfide) as the conditioned stimulus (CS) were simultaneously inhaled. After the animals were intraperitoneally injected with saline or diazepam (2.5 or 5 mg/kg), they underwent exposure to the CS and blood collecting. This procedure was repeated three times in order that the animals would have each kind of injection. The animals injected with saline showed significantly higher levels of plasma histamine following the exposure to the CS as a conditioning effect compared with the baselines (P<0.05), whereas the group injected with diazepam (5 mg/kg) did not indicate such elevations. The suppressing effect of diazepam on the conditioned histamine release was also confirmed by a multiple regression analysis (5 mg/kg) and an analysis of covariance (2.5 and 5 mg/kg), even after adjustments for several factors regarding immunological sensitization and conditionability. The present study suggests that diazepam attenuates a conditioned histamine release.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalInternational Journal of Psychophysiology
Volume51
Issue number3
DOIs
Publication statusPublished - Jan 1 2004

Fingerprint

Histamine Release
Diazepam
Guinea Pigs
Ovalbumin
Classical Conditioning
Histamine
Immunization
Asthma
Regression Analysis
Pharmacology
Antigens
Injections
Conditioning (Psychology)
Odorants

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology
  • Physiology (medical)

Cite this

Diazepam attenuates conditioned histamine release in guinea pigs. / Irie, Masahiro; Nagata, Shoji; Endo, Yutaka.

In: International Journal of Psychophysiology, Vol. 51, No. 3, 01.01.2004, p. 231-238.

Research output: Contribution to journalArticle

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