Dibutyryl cyclic adenosine monophosphate protects mice against tumor necrosis factor-α-induced hepatocyte apoptosis accompanied by increased heat shock protein 70 expression

Manabu Takano, Toshiyuki Arai, Yasuji Mokuno, Hitoshi Nishimura, Yuji Nimura, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Liver injury accompanied by apoptosis of hepatocytes was provoked in mice by an intravenous injection of recombinant tumor necrosis factor-α (rTNF-α) (1.0 μg/kg) together with an intraperitoneal injection of D-galactosamine (D-gal) (500 mg/kg). Injection of various doses of dibutyryl cAMP (DBcAMP) protected mice from TNF-α/D-gal-induced liver injury as assessed by serum alanine aminotransferase (ALT) levels, histological examination and DNA fragmentation. DBcAMP significantly enhanced the Hsp70 expression in the hepatocytes of D-gal/TNF-α-injected mice in close correlation with suppression of liver injury. DBcAMP induced Hsp70 expression in the hepatocyte in vitro. These results suggest that increase in Hsp70 expression by DBcAMP is involved in protective mechanisms by DBcAMP against TNF-α-induced liver injury in D-gal-sensitized mice.

    Original languageEnglish
    Pages (from-to)109-117
    Number of pages9
    JournalCell Stress and Chaperones
    Volume3
    Issue number2
    DOIs
    Publication statusPublished - Jun 1 1998

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Cell Biology

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