Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths

Zhenhao Ding, Hiromichi Kasahara, Michihiko Nakano, Junya Suehiro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Polymerase chain reaction (PCR) is one of the most sensitive and specific detection methods of bacterial and viral infections. The authors proposed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labelling. DNA labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. DEP-trapped microbeads can be measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. In this study, it was aimed to reveal how DNA length affects DEP characteristic of DNA-labeled microbeads. Dielectrophoretic crossover from the negative to the positive was measured for microbeads labeled with DNA length in 204 bp, 391 bp and 796 bp. After theoretical fitting of DEP crossover data, it was revealed that the surface conductance increased when the length of labeled DNA increased.

Original languageEnglish
Title of host publicationBIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015
EditorsAlberto Cliquet, Ana Fred, Hugo Gamboa, Dirk Elias, Alberto Cliquet
PublisherSciTePress
Pages185-189
Number of pages5
ISBN (Electronic)9789897580710
Publication statusPublished - Jan 1 2015
Event8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015 - Lisbon, Portugal
Duration: Jan 12 2015Jan 15 2015

Publication series

NameBIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015

Other

Other8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015
CountryPortugal
CityLisbon
Period1/12/151/15/15

Fingerprint

DNA
Electrophoresis
Polymerase chain reaction
Microelectrodes
Labeling

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

Ding, Z., Kasahara, H., Nakano, M., & Suehiro, J. (2015). Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. In A. Cliquet, A. Fred, H. Gamboa, D. Elias, & A. Cliquet (Eds.), BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015 (pp. 185-189). (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015). SciTePress.

Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. / Ding, Zhenhao; Kasahara, Hiromichi; Nakano, Michihiko; Suehiro, Junya.

BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. ed. / Alberto Cliquet; Ana Fred; Hugo Gamboa; Dirk Elias; Alberto Cliquet. SciTePress, 2015. p. 185-189 (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ding, Z, Kasahara, H, Nakano, M & Suehiro, J 2015, Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. in A Cliquet, A Fred, H Gamboa, D Elias & A Cliquet (eds), BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015, SciTePress, pp. 185-189, 8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015, Lisbon, Portugal, 1/12/15.
Ding Z, Kasahara H, Nakano M, Suehiro J. Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. In Cliquet A, Fred A, Gamboa H, Elias D, Cliquet A, editors, BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. SciTePress. 2015. p. 185-189. (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).
Ding, Zhenhao ; Kasahara, Hiromichi ; Nakano, Michihiko ; Suehiro, Junya. / Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. editor / Alberto Cliquet ; Ana Fred ; Hugo Gamboa ; Dirk Elias ; Alberto Cliquet. SciTePress, 2015. pp. 185-189 (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).
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