Dietary egg white protein inhibits lymphatic lipid transport in thoracic lymph duct-cannulated rats

Ryosuke Matsuoka, Bungo Shirouchi, Sayaka Kawamura, Sanae Baba, Sawako Shiratake, Kazuko Nagata, Katsumi Imaizumi, Masao Sato

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Dietary egg white protein (EWP) decreases serum cholesterol levels. We previously showed that EWP decreased cholesterol absorption in the intestine. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of dietary EWP on lipid transport. They were fed diets with 20% EWP and casein, and their lymph was collected to quantify lymphatic lipid levels. Dietary EWP decreased lymphatic cholesterol transport compared with casein. It was previously shown that EWP excluded cholesterol from bile acid micelles. Therefore, pepsin-hydrolyzed EWP and casein were prepared. EWP was not completely digested. Ovalbumin, which is the most abundant protein in EWP, showed resistance to digestion by pepsin. This study investigated the effects of EWP pepsin hydrolysate (EWP-ph) on cholesterol micellar solubility, cholesterol transfer from the micellar to the oil phase, water-holding capacity (WHC), settling volume in water (SV), and relative viscosity and compared them with the effects of casein pepsin hydrolysate (C-ph). EWP-ph significantly decreased the micellar solubility and transfer rate and increased the WHC, SV, and relative viscosity compared with C-ph. Moreover, the pepsin hydrolysate of ovalbumin, a major protein in EWP, played a role in decreasing cholesterol micellar solubility, leading to the inhibition of cholesterol absorption. In conclusion, dietary EWP decreased cholesterol intestinal absorption by exerting combined effects of these physicochemical properties in the gut.

Original languageEnglish
Pages (from-to)10694-10700
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number44
DOIs
Publication statusPublished - Nov 5 2014

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Dietary Egg Proteins
Egg Proteins
Thoracic Duct
lymph
Lymph
chest
egg albumen
Ducts
Rats
Pepsin A
Cholesterol
Lipids
rats
lipids
cholesterol
pepsin
Caseins
proteins
Solubility
casein

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dietary egg white protein inhibits lymphatic lipid transport in thoracic lymph duct-cannulated rats. / Matsuoka, Ryosuke; Shirouchi, Bungo; Kawamura, Sayaka; Baba, Sanae; Shiratake, Sawako; Nagata, Kazuko; Imaizumi, Katsumi; Sato, Masao.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 44, 05.11.2014, p. 10694-10700.

Research output: Contribution to journalArticle

Matsuoka, Ryosuke ; Shirouchi, Bungo ; Kawamura, Sayaka ; Baba, Sanae ; Shiratake, Sawako ; Nagata, Kazuko ; Imaizumi, Katsumi ; Sato, Masao. / Dietary egg white protein inhibits lymphatic lipid transport in thoracic lymph duct-cannulated rats. In: Journal of Agricultural and Food Chemistry. 2014 ; Vol. 62, No. 44. pp. 10694-10700.
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