Differences in interleukin-12 and -15 production by dendritic-cells at the early stage of Listeria monocytogenes infection between BALB/c and C57 BL/6 mice

Tie Liu, Hitoshi Nishimura, Tetsuya Matsuguchi, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    The mechanisms responsible for the resistance of C57BL/6 mice and for the susceptibility of BALB/c mice to infection with Listeria monocytogenes were studied by comparing early IL-12 and IL-15 production by dendritic cells (DC) after infection with L. monocytogenes. Splenic DC expressing CD11b(low) and CD11c+ obtained from C57BL/6 mice at 3 and 6 h after L. monocytogenes infection expressed higher levels of IL-12 p40 mRNA and IL-12 p40 protein than did those from BALB/c mice. Concurrently, a larger amount of IFN-γ was produced by the splenic T cells from C57BL/6 mice in response to immobilized anti-TCRαβ mAb than by those from BALB/c mice, while the splenic T cells from BALB/c mice produced a higher level of IL-4 upon TCR αβ stimulation than did those of C57BL/6 mice. IL-15 mRNA and intracellular IL-15 protein were detected more abundantly in the DC from C57BL/6 mice than in those from BALB/c mice on day 3 after infection. CD3+ IL2Rβ + cells in the spleen were increased in C57BL/6 mice but not in BALB/c mice at the early stage after infection. Furthermore, IL-12Rβ2 gene expression was up-regulated in T cells from C57BL/6 mice but not in those from BALB/c mice at the early stage after listerial infection. These results suggest that the difference in early production of IL-12 and IL-15 by DC may at least partly underlie the difference in susceptibility to L. monocytogenes between C57BL/6 and BALB/c mice. (C) 2000 Academic Press.

    Original languageEnglish
    Pages (from-to)31-40
    Number of pages10
    JournalCellular Immunology
    Volume202
    Issue number1
    DOIs
    Publication statusPublished - May 25 2000

    All Science Journal Classification (ASJC) codes

    • Immunology

    Fingerprint Dive into the research topics of 'Differences in interleukin-12 and -15 production by dendritic-cells at the early stage of Listeria monocytogenes infection between BALB/c and C57 BL/6 mice'. Together they form a unique fingerprint.

  • Cite this