Objective: The aim of this study was to compare the relationships and risk factors with hypertension, diabetes, and proteinuria between urban office workers at Dhaka city and rural community residents at Bheramara sub-district who participated in a mobile health check-up service called portable health clinic (PHC). Methods: Data were collected from 271 urban office workers at Dhaka city in 2018 and 2,890 rural community residents at Bheramara sub-district between 2013 and 2016, who agreed to participate in the PHC. Data included basic socio-demographic and health check-up information. Descriptive statistics were conducted to compare the relationships with three main outcome variables (hypertension, diabetes, and proteinuria) and independent variables (ex, age, sex, pulse rates, and body mass index (BMI)). Results: The percentage of participants who were overweight or obese was higher among urban participants (51%) than rural participants (35%), whereas in rural participants, the percentage of being underweight was higher (11%) than in urban participants (0.4%). Among participants who had proteinuria, 60% in urban and 34% in rural participants had hypertension and 10% in urban and 14% in rural and had diabetes. Among those who had diabetes, 26% in urban and 45% in rural participants had hypertension and 3% in urban and 55% in rural participants had proteinuria. Among participants who had hypertension, 9% in urban and 37% in rural participants had proteinuria 12% in both urban and rural participants had diabetes. Conclusions: Hypertension, diabetes, and proteinuria were highly co-existed particularly among rural participants. Obesity is more prevalent among urban participants. PHC services is important for screening a large number of unaware and undiagnosed diabetic, hypertensive, and proteinuria patients both in rural and urban Bangladesh.