Differences in the expression of epithelial-mesenchymal transition related molecules between primary tumors and pulmonary metastatic tumors in colorectal cancer

Hidenori Kouso, Tokujiro Yano, Riichiroh Maruyama, Yasunori Shikada, Tatsuro Okamoto, Akira Haro, Yoshihiro Kakeji, Yoshihiko Maehara

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Abstract

Purpose: Epithelial-mesenchymal transition (EMT) is a key event in cancer metastasis. This study immunohistochemically examined the expression of EMT-related molecules in both primary colorectal cancer and pulmonary metastases, and analyzed the expression pattern. Methods: Ten patients with colorectal cancer that underwent surgical resections for both the primary tumor and metastatic pulmonary tumors were included. The expression status of EMT-related molecules was examined using immunohistochemical staining. Results: Nine of the 10 cases maintained the expression of both E-cadherin and β-catenin in the primary site. The expression of E-cadherin and β-catenin in the pulmonary metastatic site was preserved in 10 and 12 out of 15 metastatic lesions, respectively. The EMT-related transcription factor, Twist, was positively expressed in all 10 cases, Smad interacting protein 1 (Sip1) in 9, Snail in 4 and Slug in 3 of the primary sites. On the other hand, staining for Twist, Sip1 and Snail at the metastatic pulmonary site, was negative in all 10 cases. Conclusion: The expression of EMT-related transcription factors in metastatic pulmonary tumors from colorectal cancer decreased in comparison to the primary tumors. These findings suggested that the expression status of EMT-related transcription factors might play an important role in the implantation of metastatic foci.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalSurgery today
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Epithelial-Mesenchymal Transition
Colorectal Neoplasms
Lung
Smad Proteins
Catenins
Neoplasms
Cadherins
Twist-Related Protein 1
Transcription Factors
Staining and Labeling
Neoplasm Metastasis
Gastropoda

All Science Journal Classification (ASJC) codes

  • Surgery

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Differences in the expression of epithelial-mesenchymal transition related molecules between primary tumors and pulmonary metastatic tumors in colorectal cancer. / Kouso, Hidenori; Yano, Tokujiro; Maruyama, Riichiroh; Shikada, Yasunori; Okamoto, Tatsuro; Haro, Akira; Kakeji, Yoshihiro; Maehara, Yoshihiko.

In: Surgery today, Vol. 43, No. 1, 01.01.2013, p. 73-80.

Research output: Contribution to journalArticle

Kouso, Hidenori ; Yano, Tokujiro ; Maruyama, Riichiroh ; Shikada, Yasunori ; Okamoto, Tatsuro ; Haro, Akira ; Kakeji, Yoshihiro ; Maehara, Yoshihiko. / Differences in the expression of epithelial-mesenchymal transition related molecules between primary tumors and pulmonary metastatic tumors in colorectal cancer. In: Surgery today. 2013 ; Vol. 43, No. 1. pp. 73-80.
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