To elucidate the relative genetic influence of Ki-Ras on the MHC class 1-bound peptide repertoire in colon cancer, and the differences in class I peptide repertoires among cells from different tissues, we isolated the peptides bound to HLA A.2.1 molecules from a colon carcinoma cell line (HTC116), a Ki-Ras gene-targeted progeny cell line (Hke-3) and an EBV-transformed B-cell line. We then analyzed the cluted peptides by tandem mass spectrometry. Although the two colon carcinoma cell lines have very different metabolisms and morphologies, due to the presence or absence of activated Ki-Ras, there were no observed differences in the MHC class I peptide repertoires between these cells. However, there were profound differences between the repertoires of the colon cancer cell lines and the B-cell line. 3 of 7 peptide CID spectra were different between B-cells and colon cancer cells, indicating that tissue differences have more influence on MHC class I repertoire than metabolic changes due to the influence of the Ki-Ras oncogene.
|Number of pages||1|
|Journal||Japanese Journal of Human Genetics|
|Publication status||Published - Dec 1 1996|
All Science Journal Classification (ASJC) codes