Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice

Masashi Emoto, Toshio Naito, Reiko Nakamura, Yasunobu Yoshikai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Ity gene, which is believed to be a single gene on proximal mouse chromosome 1, determines susceptibility to Salmonella infection. We have recently demonstrated that the γδT cells specific for purified protein derivative appear at the early stage after infection with Salmonella choleraesuis strain 31N-1 (avirulent strain) in mice. In the present study, we compared the appearance of the γδT cells in Itr and Its mice after infection with S. choleraesuis in an attempt to elucidate the role of the γδT cells in the in vivo effect of the Ity locus on murine salmonellosis. The numbers of γδ T cells in the peritoneal cavity and liver were significantly larger in DBA/2 (Ityr) mice than those in BALB/c (Itys) mice after infection with a sublethal dose of S. choleraesuis. C.D2 Ityr mice, which are BALB/c mice with Ityr allele, also showed a significant increase in the number of the γδ T cells at the early stage after infection. Similar results in different appearances of the γδ T cells could be obtained between BALB/c and DBA/2 mice after infection with Mycobacterium bovis, bacillus Calmette-Guérin. The peritoneal adherent cells of DBA/2 mice infected with S. choleraesuis, which expressed a large amount of heat shock/stress protein (hsp) homologous to the mycobacterial 65-kDa hsp, induced significantly higher level of expansion of the peritoneal γδ T cells in normal mice in vivo and in vitro than did those of BALB/c mice infected with S. choleraesuis, which expressed a lesser amount of the hsp. These results suggest that the Ity locus may be closely related to the expression of hsp by macrophages after infection with Salmonella, which in turn stimulate the γδ T cells in the host during an early phase of salmonellosis.

Original languageEnglish
Pages (from-to)3411-3420
Number of pages10
JournalJournal of Immunology
Volume150
Issue number8 PART 1
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Salmonella Infections
Heat-Shock Proteins
T-Lymphocytes
Inbred DBA Mouse
Infection
Chromosomes, Human, Pair 1
Peritoneal Cavity
Mycobacterium bovis
Bacillus
Genes
Alleles
Macrophages
Liver

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Emoto, M., Naito, T., Nakamura, R., & Yoshikai, Y. (1993). Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice. Journal of Immunology, 150(8 PART 1), 3411-3420.

Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice. / Emoto, Masashi; Naito, Toshio; Nakamura, Reiko; Yoshikai, Yasunobu.

In: Journal of Immunology, Vol. 150, No. 8 PART 1, 1993, p. 3411-3420.

Research output: Contribution to journalArticle

Emoto, M, Naito, T, Nakamura, R & Yoshikai, Y 1993, 'Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice', Journal of Immunology, vol. 150, no. 8 PART 1, pp. 3411-3420.
Emoto M, Naito T, Nakamura R, Yoshikai Y. Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice. Journal of Immunology. 1993;150(8 PART 1):3411-3420.
Emoto, Masashi ; Naito, Toshio ; Nakamura, Reiko ; Yoshikai, Yasunobu. / Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice. In: Journal of Immunology. 1993 ; Vol. 150, No. 8 PART 1. pp. 3411-3420.
@article{25e9a32015ec4a16948a052c3ae75390,
title = "Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice",
abstract = "Ity gene, which is believed to be a single gene on proximal mouse chromosome 1, determines susceptibility to Salmonella infection. We have recently demonstrated that the γδT cells specific for purified protein derivative appear at the early stage after infection with Salmonella choleraesuis strain 31N-1 (avirulent strain) in mice. In the present study, we compared the appearance of the γδT cells in Itr and Its mice after infection with S. choleraesuis in an attempt to elucidate the role of the γδT cells in the in vivo effect of the Ity locus on murine salmonellosis. The numbers of γδ T cells in the peritoneal cavity and liver were significantly larger in DBA/2 (Ityr) mice than those in BALB/c (Itys) mice after infection with a sublethal dose of S. choleraesuis. C.D2 Ityr mice, which are BALB/c mice with Ityr allele, also showed a significant increase in the number of the γδ T cells at the early stage after infection. Similar results in different appearances of the γδ T cells could be obtained between BALB/c and DBA/2 mice after infection with Mycobacterium bovis, bacillus Calmette-Gu{\'e}rin. The peritoneal adherent cells of DBA/2 mice infected with S. choleraesuis, which expressed a large amount of heat shock/stress protein (hsp) homologous to the mycobacterial 65-kDa hsp, induced significantly higher level of expansion of the peritoneal γδ T cells in normal mice in vivo and in vitro than did those of BALB/c mice infected with S. choleraesuis, which expressed a lesser amount of the hsp. These results suggest that the Ity locus may be closely related to the expression of hsp by macrophages after infection with Salmonella, which in turn stimulate the γδ T cells in the host during an early phase of salmonellosis.",
author = "Masashi Emoto and Toshio Naito and Reiko Nakamura and Yasunobu Yoshikai",
year = "1993",
language = "English",
volume = "150",
pages = "3411--3420",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8 PART 1",

}

TY - JOUR

T1 - Different appearance of γδ T cells during salmonellosis between Ityr and Itys mice

AU - Emoto, Masashi

AU - Naito, Toshio

AU - Nakamura, Reiko

AU - Yoshikai, Yasunobu

PY - 1993

Y1 - 1993

N2 - Ity gene, which is believed to be a single gene on proximal mouse chromosome 1, determines susceptibility to Salmonella infection. We have recently demonstrated that the γδT cells specific for purified protein derivative appear at the early stage after infection with Salmonella choleraesuis strain 31N-1 (avirulent strain) in mice. In the present study, we compared the appearance of the γδT cells in Itr and Its mice after infection with S. choleraesuis in an attempt to elucidate the role of the γδT cells in the in vivo effect of the Ity locus on murine salmonellosis. The numbers of γδ T cells in the peritoneal cavity and liver were significantly larger in DBA/2 (Ityr) mice than those in BALB/c (Itys) mice after infection with a sublethal dose of S. choleraesuis. C.D2 Ityr mice, which are BALB/c mice with Ityr allele, also showed a significant increase in the number of the γδ T cells at the early stage after infection. Similar results in different appearances of the γδ T cells could be obtained between BALB/c and DBA/2 mice after infection with Mycobacterium bovis, bacillus Calmette-Guérin. The peritoneal adherent cells of DBA/2 mice infected with S. choleraesuis, which expressed a large amount of heat shock/stress protein (hsp) homologous to the mycobacterial 65-kDa hsp, induced significantly higher level of expansion of the peritoneal γδ T cells in normal mice in vivo and in vitro than did those of BALB/c mice infected with S. choleraesuis, which expressed a lesser amount of the hsp. These results suggest that the Ity locus may be closely related to the expression of hsp by macrophages after infection with Salmonella, which in turn stimulate the γδ T cells in the host during an early phase of salmonellosis.

AB - Ity gene, which is believed to be a single gene on proximal mouse chromosome 1, determines susceptibility to Salmonella infection. We have recently demonstrated that the γδT cells specific for purified protein derivative appear at the early stage after infection with Salmonella choleraesuis strain 31N-1 (avirulent strain) in mice. In the present study, we compared the appearance of the γδT cells in Itr and Its mice after infection with S. choleraesuis in an attempt to elucidate the role of the γδT cells in the in vivo effect of the Ity locus on murine salmonellosis. The numbers of γδ T cells in the peritoneal cavity and liver were significantly larger in DBA/2 (Ityr) mice than those in BALB/c (Itys) mice after infection with a sublethal dose of S. choleraesuis. C.D2 Ityr mice, which are BALB/c mice with Ityr allele, also showed a significant increase in the number of the γδ T cells at the early stage after infection. Similar results in different appearances of the γδ T cells could be obtained between BALB/c and DBA/2 mice after infection with Mycobacterium bovis, bacillus Calmette-Guérin. The peritoneal adherent cells of DBA/2 mice infected with S. choleraesuis, which expressed a large amount of heat shock/stress protein (hsp) homologous to the mycobacterial 65-kDa hsp, induced significantly higher level of expansion of the peritoneal γδ T cells in normal mice in vivo and in vitro than did those of BALB/c mice infected with S. choleraesuis, which expressed a lesser amount of the hsp. These results suggest that the Ity locus may be closely related to the expression of hsp by macrophages after infection with Salmonella, which in turn stimulate the γδ T cells in the host during an early phase of salmonellosis.

UR - http://www.scopus.com/inward/record.url?scp=0027191217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027191217&partnerID=8YFLogxK

M3 - Article

C2 - 8468480

AN - SCOPUS:0027191217

VL - 150

SP - 3411

EP - 3420

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8 PART 1

ER -