Different effects of trypsin inhibitors on intestinal gene expression of secretin and on pancreatic bicarbonate secretion in CCK-A-receptor-deficient rats

Takako Kawanami, Shinji Suzuki, Yuki Yoshida, Setsuko Kanai, Yutaka Takata, Takao Shimazoe, Shigenori Watanabe, Akihiro Funakoshi, Kyoko Miyasaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of oral administration of two synthetic trypsin inhibitors (camostate and ONO-3403) and soybean trypsin inhibitor (SBTI) on cholecystokinin (CCK), secretin gene expression and pancreatic secretion were examined in CCK-A-receptor-deficient (OLETF) rats. The rats were fed chow containing 0.1% trypsin inhibitors for 7 days. To examine pancreatic secretion, the rats were prepared with cannulae to drain the bile and pancreatic juice separately, a duodenal cannula and an external jugular vein cannula. The animals were maintained in Bollman cages and the experiments were conducted 4 days after surgery. The levels of CCK mRNA were significantly increased by each treatment. The levels of secretin mRNA were significantly increased by camostate and SBTI, but not by ONO-3403. Bicarbonate secretion was significantly increased in rats treated with camostate and ONO-3403, but not SBTI, while protein secretion was not affected by any treatment. These observations suggest that increased bicarbonate secretion produced by synthetic trypsin inhibitors in CCK-A-receptor-deficient rats may not be due to secretin but due to ONO-3403 in the circulation.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalJapanese Journal of Pharmacology
Volume81
Issue number4
DOIs
Publication statusPublished - Dec 1 1999

Fingerprint

Cholecystokinin A Receptor
Secretin
Trypsin Inhibitors
Bicarbonates
Gene Expression
Soybeans
Cholecystokinin
Inbred OLETF Rats
Pancreatic Juice
Messenger RNA
Jugular Veins
Ambulatory Surgical Procedures
Bile
Oral Administration
ethyl N-allyl-N-(2-methyl-3-(4-(4-amidinophenoxycarbonyl)phenyl)propenoyl)aminoacetate methanesulfonate
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Different effects of trypsin inhibitors on intestinal gene expression of secretin and on pancreatic bicarbonate secretion in CCK-A-receptor-deficient rats. / Kawanami, Takako; Suzuki, Shinji; Yoshida, Yuki; Kanai, Setsuko; Takata, Yutaka; Shimazoe, Takao; Watanabe, Shigenori; Funakoshi, Akihiro; Miyasaka, Kyoko.

In: Japanese Journal of Pharmacology, Vol. 81, No. 4, 01.12.1999, p. 339-345.

Research output: Contribution to journalArticle

Kawanami, Takako ; Suzuki, Shinji ; Yoshida, Yuki ; Kanai, Setsuko ; Takata, Yutaka ; Shimazoe, Takao ; Watanabe, Shigenori ; Funakoshi, Akihiro ; Miyasaka, Kyoko. / Different effects of trypsin inhibitors on intestinal gene expression of secretin and on pancreatic bicarbonate secretion in CCK-A-receptor-deficient rats. In: Japanese Journal of Pharmacology. 1999 ; Vol. 81, No. 4. pp. 339-345.
@article{bb46b9257fa64ad4acb85acf074f81a9,
title = "Different effects of trypsin inhibitors on intestinal gene expression of secretin and on pancreatic bicarbonate secretion in CCK-A-receptor-deficient rats",
abstract = "The effects of oral administration of two synthetic trypsin inhibitors (camostate and ONO-3403) and soybean trypsin inhibitor (SBTI) on cholecystokinin (CCK), secretin gene expression and pancreatic secretion were examined in CCK-A-receptor-deficient (OLETF) rats. The rats were fed chow containing 0.1{\%} trypsin inhibitors for 7 days. To examine pancreatic secretion, the rats were prepared with cannulae to drain the bile and pancreatic juice separately, a duodenal cannula and an external jugular vein cannula. The animals were maintained in Bollman cages and the experiments were conducted 4 days after surgery. The levels of CCK mRNA were significantly increased by each treatment. The levels of secretin mRNA were significantly increased by camostate and SBTI, but not by ONO-3403. Bicarbonate secretion was significantly increased in rats treated with camostate and ONO-3403, but not SBTI, while protein secretion was not affected by any treatment. These observations suggest that increased bicarbonate secretion produced by synthetic trypsin inhibitors in CCK-A-receptor-deficient rats may not be due to secretin but due to ONO-3403 in the circulation.",
author = "Takako Kawanami and Shinji Suzuki and Yuki Yoshida and Setsuko Kanai and Yutaka Takata and Takao Shimazoe and Shigenori Watanabe and Akihiro Funakoshi and Kyoko Miyasaka",
year = "1999",
month = "12",
day = "1",
doi = "10.1254/jjp.81.339",
language = "English",
volume = "81",
pages = "339--345",
journal = "Japanese Journal of Pharmacology",
issn = "0021-5198",
publisher = "Japanese Pharmacological Society",
number = "4",

}

TY - JOUR

T1 - Different effects of trypsin inhibitors on intestinal gene expression of secretin and on pancreatic bicarbonate secretion in CCK-A-receptor-deficient rats

AU - Kawanami, Takako

AU - Suzuki, Shinji

AU - Yoshida, Yuki

AU - Kanai, Setsuko

AU - Takata, Yutaka

AU - Shimazoe, Takao

AU - Watanabe, Shigenori

AU - Funakoshi, Akihiro

AU - Miyasaka, Kyoko

PY - 1999/12/1

Y1 - 1999/12/1

N2 - The effects of oral administration of two synthetic trypsin inhibitors (camostate and ONO-3403) and soybean trypsin inhibitor (SBTI) on cholecystokinin (CCK), secretin gene expression and pancreatic secretion were examined in CCK-A-receptor-deficient (OLETF) rats. The rats were fed chow containing 0.1% trypsin inhibitors for 7 days. To examine pancreatic secretion, the rats were prepared with cannulae to drain the bile and pancreatic juice separately, a duodenal cannula and an external jugular vein cannula. The animals were maintained in Bollman cages and the experiments were conducted 4 days after surgery. The levels of CCK mRNA were significantly increased by each treatment. The levels of secretin mRNA were significantly increased by camostate and SBTI, but not by ONO-3403. Bicarbonate secretion was significantly increased in rats treated with camostate and ONO-3403, but not SBTI, while protein secretion was not affected by any treatment. These observations suggest that increased bicarbonate secretion produced by synthetic trypsin inhibitors in CCK-A-receptor-deficient rats may not be due to secretin but due to ONO-3403 in the circulation.

AB - The effects of oral administration of two synthetic trypsin inhibitors (camostate and ONO-3403) and soybean trypsin inhibitor (SBTI) on cholecystokinin (CCK), secretin gene expression and pancreatic secretion were examined in CCK-A-receptor-deficient (OLETF) rats. The rats were fed chow containing 0.1% trypsin inhibitors for 7 days. To examine pancreatic secretion, the rats were prepared with cannulae to drain the bile and pancreatic juice separately, a duodenal cannula and an external jugular vein cannula. The animals were maintained in Bollman cages and the experiments were conducted 4 days after surgery. The levels of CCK mRNA were significantly increased by each treatment. The levels of secretin mRNA were significantly increased by camostate and SBTI, but not by ONO-3403. Bicarbonate secretion was significantly increased in rats treated with camostate and ONO-3403, but not SBTI, while protein secretion was not affected by any treatment. These observations suggest that increased bicarbonate secretion produced by synthetic trypsin inhibitors in CCK-A-receptor-deficient rats may not be due to secretin but due to ONO-3403 in the circulation.

UR - http://www.scopus.com/inward/record.url?scp=0033370025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033370025&partnerID=8YFLogxK

U2 - 10.1254/jjp.81.339

DO - 10.1254/jjp.81.339

M3 - Article

VL - 81

SP - 339

EP - 345

JO - Japanese Journal of Pharmacology

JF - Japanese Journal of Pharmacology

SN - 0021-5198

IS - 4

ER -