TY - JOUR
T1 - Different expression systems resulted in varied binding properties of anti-paclitaxel single-chain variable fragment antibody clone 1C2
AU - Yusakul, Gorawit
AU - Sakamoto, Seiichi
AU - Nuntawong, Poomraphie
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
N1 - Funding Information:
Acknowledgements We acknowledge financial support from the Japan Society for the Promotion of Science (No. 22501046 and No. 19590119) and from the Takeda Science Foundation. We also appreciate the technical support from the Research Support Center, Graduate School of Medical Sciences, Kyushu University, Japan.
Publisher Copyright:
© 2017, The Japanese Society of Pharmacognosy and Springer Japan KK.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The binding properties of recombinant antibody fragments, such as affinity and specificity, determine their usefulness for therapeutic and analytical applications. Anti-paclitaxel single-chain variable fragment clone 1C2 (anti-PT scFv1C2) was expressed using Escherichia coli cell and Bombyx mori larvae expression systems. The binding characteristics of the scFvs were evaluated using indirect competitive ELISA. The linear range of binding between anti-PT scFv1C2 and paclitaxel was significantly different between the anti-PT scFv1C2s derived from E. coli (1.056–5.700 μg/ml) and B. mori (7.813–1000 ng/ml), which indicated that different expression systems resulted in different sensitivities for paclitaxel determination. In addition, the binding specificity of anti-PT scFv1C2 varied between expression systems. This finding implied that the expression system significantly affects the binding properties of recombinant antibodies, especially antibodies against low-molecular-weight targets.
AB - The binding properties of recombinant antibody fragments, such as affinity and specificity, determine their usefulness for therapeutic and analytical applications. Anti-paclitaxel single-chain variable fragment clone 1C2 (anti-PT scFv1C2) was expressed using Escherichia coli cell and Bombyx mori larvae expression systems. The binding characteristics of the scFvs were evaluated using indirect competitive ELISA. The linear range of binding between anti-PT scFv1C2 and paclitaxel was significantly different between the anti-PT scFv1C2s derived from E. coli (1.056–5.700 μg/ml) and B. mori (7.813–1000 ng/ml), which indicated that different expression systems resulted in different sensitivities for paclitaxel determination. In addition, the binding specificity of anti-PT scFv1C2 varied between expression systems. This finding implied that the expression system significantly affects the binding properties of recombinant antibodies, especially antibodies against low-molecular-weight targets.
UR - http://www.scopus.com/inward/record.url?scp=85031400722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85031400722&partnerID=8YFLogxK
U2 - 10.1007/s11418-017-1136-z
DO - 10.1007/s11418-017-1136-z
M3 - Article
C2 - 29027080
AN - SCOPUS:85031400722
SN - 1340-3443
VL - 72
SP - 310
EP - 316
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 1
ER -