Different profile of interleukin-10 production in circulating T cells from atopic asthmatics compared with healthy subjects

K. Matsumoto, S. Narita, T. Rerecich, D. P. Snider, P. M. O'Byrne

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: Interleukin (IL)-10 is a pleiotropic cytokine released from various cells, including T cells. Although IL-10 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL-10 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls. Methods: Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and CD4/CD8 antibodies, and then processed for intracellular IL-10 detection by flow cytometry. Results: A kinetic study in healthy controls showed that stimulation with phorbol 12-myristate 13-acetate and ionomycin significantly increased the frequencies of IL-10-producing CD3+, CD4 + and CD8+ cells. Without stimulation, the frequencies of IL-10-producing CD3+, CD4+ and CD8 + cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL-10-producing CD3+, CD4+ and CD8+ cells in healthy controls and atopic nonasthmatics, but not in asthmatics. Conclusions: The frequency of IL-10-producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12-myristate 13-acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL-10 production; alternatively, IL-10 production by T cells from asthmatics may be regulated differently than T cells from other subjects.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalCanadian Respiratory Journal
Volume11
Issue number1
DOIs
Publication statusPublished - 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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