Abstract
Background: Interleukin (IL)-10 is a pleiotropic cytokine released from various cells, including T cells. Although IL-10 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL-10 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls. Methods: Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and CD4/CD8 antibodies, and then processed for intracellular IL-10 detection by flow cytometry. Results: A kinetic study in healthy controls showed that stimulation with phorbol 12-myristate 13-acetate and ionomycin significantly increased the frequencies of IL-10-producing CD3+, CD4 + and CD8+ cells. Without stimulation, the frequencies of IL-10-producing CD3+, CD4+ and CD8 + cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL-10-producing CD3+, CD4+ and CD8+ cells in healthy controls and atopic nonasthmatics, but not in asthmatics. Conclusions: The frequency of IL-10-producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12-myristate 13-acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL-10 production; alternatively, IL-10 production by T cells from asthmatics may be regulated differently than T cells from other subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 33-38 |
| Number of pages | 6 |
| Journal | Canadian Respiratory Journal |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2004 |
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All Science Journal Classification (ASJC) codes
- Pulmonary and Respiratory Medicine
Cite this
Different profile of interleukin-10 production in circulating T cells from atopic asthmatics compared with healthy subjects. / Matsumoto, K.; Narita, S.; Rerecich, T.; Snider, D. P.; O'Byrne, P. M.
In: Canadian Respiratory Journal, Vol. 11, No. 1, 01.01.2004, p. 33-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different profile of interleukin-10 production in circulating T cells from atopic asthmatics compared with healthy subjects
AU - Matsumoto, K.
AU - Narita, S.
AU - Rerecich, T.
AU - Snider, D. P.
AU - O'Byrne, P. M.
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Background: Interleukin (IL)-10 is a pleiotropic cytokine released from various cells, including T cells. Although IL-10 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL-10 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls. Methods: Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and CD4/CD8 antibodies, and then processed for intracellular IL-10 detection by flow cytometry. Results: A kinetic study in healthy controls showed that stimulation with phorbol 12-myristate 13-acetate and ionomycin significantly increased the frequencies of IL-10-producing CD3+, CD4 + and CD8+ cells. Without stimulation, the frequencies of IL-10-producing CD3+, CD4+ and CD8 + cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL-10-producing CD3+, CD4+ and CD8+ cells in healthy controls and atopic nonasthmatics, but not in asthmatics. Conclusions: The frequency of IL-10-producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12-myristate 13-acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL-10 production; alternatively, IL-10 production by T cells from asthmatics may be regulated differently than T cells from other subjects.
AB - Background: Interleukin (IL)-10 is a pleiotropic cytokine released from various cells, including T cells. Although IL-10 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL-10 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls. Methods: Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and CD4/CD8 antibodies, and then processed for intracellular IL-10 detection by flow cytometry. Results: A kinetic study in healthy controls showed that stimulation with phorbol 12-myristate 13-acetate and ionomycin significantly increased the frequencies of IL-10-producing CD3+, CD4 + and CD8+ cells. Without stimulation, the frequencies of IL-10-producing CD3+, CD4+ and CD8 + cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL-10-producing CD3+, CD4+ and CD8+ cells in healthy controls and atopic nonasthmatics, but not in asthmatics. Conclusions: The frequency of IL-10-producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12-myristate 13-acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL-10 production; alternatively, IL-10 production by T cells from asthmatics may be regulated differently than T cells from other subjects.
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U2 - 10.1155/2004/710962
DO - 10.1155/2004/710962
M3 - Article
C2 - 15010730
AN - SCOPUS:1642445377
VL - 11
SP - 33
EP - 38
JO - Canadian Respiratory Journal
JF - Canadian Respiratory Journal
SN - 1198-2241
IS - 1
ER -