Different profiles of IL-10+IFN-γ-IL-4-CD4+ T cells in the peripheral blood in atopic and non-atopic asthmatics

K. Matsumoto, H. Inoue, M. Tsuda, T. Nakano, M. Komori, S. Fukuyama, Y. Nakanishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: The impaired production of interleukin (IL) 10 from regulatory T cells has been proposed as a causal mechanism of asthma. Although IL-10-producing (IL-10+) T cells are detectable in the peripheral blood, their significance in the pathophysiology of asthma remains uncertain. Objectives: This study aimed to investigate the profile of circulating IL-10+CD4+ T cells in atopic and non-atopic asthma. Methods: Atopic and non-atopic asthmatics were divided into a mild and severe group. Their peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 and anti-CD28 antibodies and then processed for triple cytokine flow cytometry directed to IL-10, interferon (IFN) γ and IL-4. Results: IL-10+CD4+ cells were exclusively detected in the IFN-γ-IL-4- population. In atopic asthma, the frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was significantly lower than that in the mild group. The frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was not significantly different from that in the mild group of those with non-atopic asthma. The frequency of IL-4+IFN-γ-IL-10-CD4+ cells (Th2) was significantly higher in the group with mild atopic asthma than in that with mild non-atopic asthma. IFN-γ+IL-4-IL-10-CD4+ cells (Th1) did not differ between groups, irrespective whether the subjects suffered from atopic or non-atopic asthma. Conclusions: IL-10+CD4+ cells in PBMCs may be distinct from Th1 or Th2 and likely have the profile of regulatory T cells. The differential association of IL-10+IFN-γ-IL-4-CD4+ cells with clinical severity between atopic and non-atopic asthma implies that its pathophysiological significance may differ among asthma phenotypes.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalRespiration
Volume75
Issue number3
DOIs
Publication statusPublished - Mar 1 2008

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Interleukin-4
Interleukin-10
Interferons
T-Lymphocytes
Asthma
Regulatory T-Lymphocytes
Blood Cells
Th2 Cells
Th1 Cells
Anti-Idiotypic Antibodies
Flow Cytometry
Cytokines
Phenotype

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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Different profiles of IL-10+IFN-γ-IL-4-CD4+ T cells in the peripheral blood in atopic and non-atopic asthmatics. / Matsumoto, K.; Inoue, H.; Tsuda, M.; Nakano, T.; Komori, M.; Fukuyama, S.; Nakanishi, Y.

In: Respiration, Vol. 75, No. 3, 01.03.2008, p. 281-287.

Research output: Contribution to journalArticle

Matsumoto, K. ; Inoue, H. ; Tsuda, M. ; Nakano, T. ; Komori, M. ; Fukuyama, S. ; Nakanishi, Y. / Different profiles of IL-10+IFN-γ-IL-4-CD4+ T cells in the peripheral blood in atopic and non-atopic asthmatics. In: Respiration. 2008 ; Vol. 75, No. 3. pp. 281-287.
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AU - Matsumoto, K.

AU - Inoue, H.

AU - Tsuda, M.

AU - Nakano, T.

AU - Komori, M.

AU - Fukuyama, S.

AU - Nakanishi, Y.

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N2 - Background: The impaired production of interleukin (IL) 10 from regulatory T cells has been proposed as a causal mechanism of asthma. Although IL-10-producing (IL-10+) T cells are detectable in the peripheral blood, their significance in the pathophysiology of asthma remains uncertain. Objectives: This study aimed to investigate the profile of circulating IL-10+CD4+ T cells in atopic and non-atopic asthma. Methods: Atopic and non-atopic asthmatics were divided into a mild and severe group. Their peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 and anti-CD28 antibodies and then processed for triple cytokine flow cytometry directed to IL-10, interferon (IFN) γ and IL-4. Results: IL-10+CD4+ cells were exclusively detected in the IFN-γ-IL-4- population. In atopic asthma, the frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was significantly lower than that in the mild group. The frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was not significantly different from that in the mild group of those with non-atopic asthma. The frequency of IL-4+IFN-γ-IL-10-CD4+ cells (Th2) was significantly higher in the group with mild atopic asthma than in that with mild non-atopic asthma. IFN-γ+IL-4-IL-10-CD4+ cells (Th1) did not differ between groups, irrespective whether the subjects suffered from atopic or non-atopic asthma. Conclusions: IL-10+CD4+ cells in PBMCs may be distinct from Th1 or Th2 and likely have the profile of regulatory T cells. The differential association of IL-10+IFN-γ-IL-4-CD4+ cells with clinical severity between atopic and non-atopic asthma implies that its pathophysiological significance may differ among asthma phenotypes.

AB - Background: The impaired production of interleukin (IL) 10 from regulatory T cells has been proposed as a causal mechanism of asthma. Although IL-10-producing (IL-10+) T cells are detectable in the peripheral blood, their significance in the pathophysiology of asthma remains uncertain. Objectives: This study aimed to investigate the profile of circulating IL-10+CD4+ T cells in atopic and non-atopic asthma. Methods: Atopic and non-atopic asthmatics were divided into a mild and severe group. Their peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 and anti-CD28 antibodies and then processed for triple cytokine flow cytometry directed to IL-10, interferon (IFN) γ and IL-4. Results: IL-10+CD4+ cells were exclusively detected in the IFN-γ-IL-4- population. In atopic asthma, the frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was significantly lower than that in the mild group. The frequency of IL-10+IFN-γ-IL-4-CD4+ cells in the severe group was not significantly different from that in the mild group of those with non-atopic asthma. The frequency of IL-4+IFN-γ-IL-10-CD4+ cells (Th2) was significantly higher in the group with mild atopic asthma than in that with mild non-atopic asthma. IFN-γ+IL-4-IL-10-CD4+ cells (Th1) did not differ between groups, irrespective whether the subjects suffered from atopic or non-atopic asthma. Conclusions: IL-10+CD4+ cells in PBMCs may be distinct from Th1 or Th2 and likely have the profile of regulatory T cells. The differential association of IL-10+IFN-γ-IL-4-CD4+ cells with clinical severity between atopic and non-atopic asthma implies that its pathophysiological significance may differ among asthma phenotypes.

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