Different vasculoprotective roles of NO synthase isoforms in vascular lesion formation in mice.

K. Yogo, H. Shimokawa, H. Funakoshi, Tadashi Kandabashi, K. Miyata, S. Okamoto, Kensuke Egashira, P. Huang, T. Akaike, A. Takeshita

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Abstract

NO is known to have several important vasculoprotective actions. Although NO is synthesized by 3 different NO synthase (NOS) isoforms, the vasculoprotective action of individual NOS isoforms remains to be clarified. Permanent ligation of the left common carotid artery was performed in control, endothelial NOS (eNOS) knockout (eNOS-KO), and inducible NOS (iNOS) knockout (iNOS-KO) mice. Four weeks after the procedure, neointimal formation and reduction of cross-sectional vascular area (constrictive remodeling) were noted in the left carotid artery. In the eNOS-KO mice, the extent of neointimal formation was significantly larger than in the control or iNOS-KO mice, whereas the extent of vascular remodeling was the highest in the iNOS-KO mice compared with other 2 strains. Antiplatelet therapy with aspirin or antihypertensive treatment with bunazosin failed to inhibit the accelerated neointimal formation in the eNOS-KO mice. These results indicate that eNOS and iNOS have different vasculoprotective actions against the vascular lesion formation caused by blood flow disruption in vivo: NO derived from eNOS inhibits neointimal formation, whereas NO derived from iNOS suppresses the development of constrictive remodeling.

Original languageEnglish
JournalArteriosclerosis, thrombosis, and vascular biology
Volume20
Issue number11
Publication statusPublished - Jan 1 2000

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Nitric Oxide Synthase
Blood Vessels
Protein Isoforms
Common Carotid Artery
Carotid Arteries
Knockout Mice
Antihypertensive Agents
Aspirin
Ligation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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Different vasculoprotective roles of NO synthase isoforms in vascular lesion formation in mice. / Yogo, K.; Shimokawa, H.; Funakoshi, H.; Kandabashi, Tadashi; Miyata, K.; Okamoto, S.; Egashira, Kensuke; Huang, P.; Akaike, T.; Takeshita, A.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, No. 11, 01.01.2000.

Research output: Contribution to journalArticle

Yogo, K, Shimokawa, H, Funakoshi, H, Kandabashi, T, Miyata, K, Okamoto, S, Egashira, K, Huang, P, Akaike, T & Takeshita, A 2000, 'Different vasculoprotective roles of NO synthase isoforms in vascular lesion formation in mice.', Arteriosclerosis, thrombosis, and vascular biology, vol. 20, no. 11.
Yogo, K. ; Shimokawa, H. ; Funakoshi, H. ; Kandabashi, Tadashi ; Miyata, K. ; Okamoto, S. ; Egashira, Kensuke ; Huang, P. ; Akaike, T. ; Takeshita, A. / Different vasculoprotective roles of NO synthase isoforms in vascular lesion formation in mice. In: Arteriosclerosis, thrombosis, and vascular biology. 2000 ; Vol. 20, No. 11.
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