Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles

Takuya Watanabe, Akira Yoshimura, Yukio Mishima, Yoshiro Endo, Toshihiko Shiroishi, Tuyoshi Koide, Hiroyuki Sasaki, Hitoshi Asakura, Ryo Kominami

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chromosomal regions subject to genomic imprinting comprise a functional domain exhibiting parental-specific expression of genes and hence may take a unique chromatin structure. Here we have examined the chromatin packaging state of allelic sites in the Zfp127/Snrpn locus on mouse chromosome 7 and in the Igf2r locus on mouse chromosome 17 with an assay consisting of chromatin fractionation and allele-specific detection. The results showed that non-transcribed alleles of Igf2r are packaged more compactly than transcribed alleles in F1 hybrid mice of both types of cross between C57BL/6 and MSM strains, whereas a non-imprinted gene, Sod-2, in the vicinity of Igf2r does not show such a difference. This indicates a close correlation between imprinting and the differential packaging of chromatin. On the other hand, the Zfp127/Snrpn locus showed such an allele-specific fractionation pattern only in F1 hybrid mice of a cross but not in those of the reciprocal cross. Analysis of the congenic mice produced for this locus did not provide any difference. These results suggest that chromatin of imprinted domains in different compaction levels is affected by distinct blueprints in homologous chromosomes that are heritable through the germ line.

Original languageEnglish
Pages (from-to)3029-3035
Number of pages7
JournalHuman Molecular Genetics
Volume9
Issue number20
Publication statusPublished - Dec 12 2000
Externally publishedYes

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Product Packaging
Chromatin
Alleles
Congenic Mice
Genomic Imprinting
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 7
Germ Cells
Chromosomes
Gene Expression
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Watanabe, T., Yoshimura, A., Mishima, Y., Endo, Y., Shiroishi, T., Koide, T., ... Kominami, R. (2000). Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles. Human Molecular Genetics, 9(20), 3029-3035.

Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles. / Watanabe, Takuya; Yoshimura, Akira; Mishima, Yukio; Endo, Yoshiro; Shiroishi, Toshihiko; Koide, Tuyoshi; Sasaki, Hiroyuki; Asakura, Hitoshi; Kominami, Ryo.

In: Human Molecular Genetics, Vol. 9, No. 20, 12.12.2000, p. 3029-3035.

Research output: Contribution to journalArticle

Watanabe, T, Yoshimura, A, Mishima, Y, Endo, Y, Shiroishi, T, Koide, T, Sasaki, H, Asakura, H & Kominami, R 2000, 'Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles', Human Molecular Genetics, vol. 9, no. 20, pp. 3029-3035.
Watanabe T, Yoshimura A, Mishima Y, Endo Y, Shiroishi T, Koide T et al. Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles. Human Molecular Genetics. 2000 Dec 12;9(20):3029-3035.
Watanabe, Takuya ; Yoshimura, Akira ; Mishima, Yukio ; Endo, Yoshiro ; Shiroishi, Toshihiko ; Koide, Tuyoshi ; Sasaki, Hiroyuki ; Asakura, Hitoshi ; Kominami, Ryo. / Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles. In: Human Molecular Genetics. 2000 ; Vol. 9, No. 20. pp. 3029-3035.
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