Differential diagnosis between complete and partial mole by TSSC3 antibody completely correlates to DNA diagnosis

Hidenori Kato, Takao Matsuda, Toshio Hirakawa, Kyoko Ueda, Takafumi Inoue, Yoko Miyanari, Kazuo Asanoma, Hitoo Nakano, Norio Wake

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Complete hydatidiform moles (CHMs) are a type ofandrogenetic fertilization without an ovum. Cases of CHM exhibit a generalized swelling of the villi and are known to be highly associated with persistent disease or carcinoma. In contrast, partial hydatidiform moles (PHMs) also show characteristic hydropic changes among the villi, but the incidence of secondary disease is relatively low. Because PHMs are fertilized by one ovum and two sperm and CHMs are fertilized by one or two sperm alone, we considered whether or not maternally imprinted genes might be useful for achieving a differential diagnosis. The validity of the imprinted genes in CHMs was assessed by implementation of a microarray technique. Among the genes examined, TSSC3, SLC22A1L, KCNQ1, and Decorin were shown to be down-regulated, and TSSC3 was the most markedly suppressed of these genes. In this study, 20 cases of CHM, the diagnosis of which was confirmed by DNA polymorphism, were investigated. In all of these cases, the expression of TSSC3 was completely absent, as determined by Western blot analysis. Conversely, 12 cases of PHM, also diagnosed by DNA polymorphism, were examined here; in all of these 12 cases, TSSC3 was found to be expressed normally. Immunohistochemical (IHC) analysis also produced the same results. The complete silencing of TSSC3 in cases of CHM will provide a novel, convenient strategy for the diagnosis of molar lesions in the placenta.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalDiagnostic Molecular Pathology
Volume14
Issue number3
DOIs
Publication statusPublished - Sep 2005

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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