Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast

Fumihito Miura, Tetsushi Yada, Kenta Nakai, Yoshiyuki Sakaki, Takashi Ito

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well-known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalFEBS Letters
Volume505
Issue number1
DOIs
Publication statusPublished - Sep 7 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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