Differential display analysis reveals the expression of glutathione S-transferase ω and novel genes through an ITAM-containing receptor in ascidian immunocytes

Kaoru Azumi, Takeshi Sasaki, Kazuki Okochi, Sho Yamasaki, Takashi Saito, Hajime Takayama, Hideyoshi Yokosawa

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The immunoreceptor tyrosine-based activation motif (ITAM) plays an important role in signal transduction through antigen receptors in mammalian lymphocytes. We previously reported that an ITAM-containing receptor, ascidian hemocyte ITAM-containing receptor 1 (AhITAMR1), exists on the hemocyte surfaces of the ascidian Halocynthia roretzi, and is involved in both phagocytosis and hemocyte aggregation. In this study, we carried out differential display screening of upregulated genes during H. roretzi hemocyte aggregation and found that at least three genes are upregulated. One encodes glutathione S-transferase ω (GSTω), while the other two encode novel proteins. The expression of all three genes was induced by treatment with a specific monoclonal antibody against AhITAMR1, while their expression was inhibited by wortmannin, BAPTA-AM, and cyclosporin A. We also found that the expression of GSTω was induced by treatment with anti-T cell receptor antibody in mouse peripheral T cells. We propose that signal transduction pathways mediated by ITAM-containing receptors are conserved from ascidian hemocytes to mammalian T cells.

    Original languageEnglish
    Pages (from-to)444-452
    Number of pages9
    JournalImmunogenetics
    Volume57
    Issue number6
    DOIs
    Publication statusPublished - Jul 1 2005

    All Science Journal Classification (ASJC) codes

    • Immunology
    • Genetics

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