Differential effects of buckwheat and kudingcha extract on neuronal damage in cultured hippocampal neurons and spatial memory impairment induced by scopolamine in an eight-arm radial maze

Fengling Pu, Kenichi Mishima, Keiichi Irie, Nobuaki Egashira, Daisuke Ishibashi, Yoshiaki Matsumoto, Tomoaki Ikeda, Katsunori Iwasaki, Hajime Fujii, Kenichi Kosuna, Michihiro Fujiwara

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We have reported the neuroprotection provided by an extract of buckwheat (BWE, Fagopyrum esculentum Moench) on neuronal damage in the repeated cerebral ischemia model and demonstrated that BWE inhibited the excess glutamate release induced by repeated cerebral ischemia, suggesting that BWE had a free radical-scavenging in addition to anti-glutamate action. In the present study, we studied the neuroprotective effects of Kudingcha extract (KDE, Ligustrum purpurascens Y. C.) on scavenging by the 2,2-diphenyl-1-picylhydrazyl (DPPH) radical in primary cultured hippocampal neurons, and on spatial memory impairment induced by scopolamine in an eight-arm radial maze in comparison with BWE. The effects of KDE (0.01-1 mg/ml) were more potent than those of BWE (0.01-1 mg/ml) in scavenging the DPPH radical (1 mM). KDE (100 μg/ml) prevented the cell damage induced by glutamate (300 μM) or kainate (1 mM), which was more potent than BWE (100 μg/ml), but BWE suppressed the cellular damage induced by β-amyloid(25-35) (20 μM) more potently than KDE (100 μg/ml). BWE (600 mg/kg), but not KDE, significantly suppressed the increase in errors induced by scopolamine (0.5 mg/kg i.p.) in the eight-arm radial maze. The results suggest that BWE may protect against cholinergic dysfunction and that KDE protects more effectively against glutaminergic dysfunction.

Original languageEnglish
Pages (from-to)636-644
Number of pages9
JournalJournal of Health Science
Volume51
Issue number6
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

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