Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice

Kazuaki Yoshinaga, Keiichi Sasaki, Hiroyuki Watanabe, Koji Nagao, Nao Inoue, Bungo Shirouchi, Teruyoshi Yanagita, Toshiharu Nagai, Hoyo Mizobe, Koichi Kojima, Fumiaki Beppu, Naohiro Gotoh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty acid synthetic enzymes and cholesterol-metabolism enzymes and thus reduced TAG and cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and cholesterol metabolism as well as eicosanoid production in C57BL/6J mice.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

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Unsaturated Fatty Acids
Inbred C57BL Mouse
Lipid Metabolism
Isomers
Triglycerides
Eicosapentaenoic Acid
Docosahexaenoic Acids
Glycerol
Cholesterol
Metabolism
Eicosanoids
Liver
Epoprostenol
Enzymes
Fatty Acids
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice. / Yoshinaga, Kazuaki; Sasaki, Keiichi; Watanabe, Hiroyuki; Nagao, Koji; Inoue, Nao; Shirouchi, Bungo; Yanagita, Teruyoshi; Nagai, Toshiharu; Mizobe, Hoyo; Kojima, Koichi; Beppu, Fumiaki; Gotoh, Naohiro.

In: Journal of Nutritional Biochemistry, Vol. 26, No. 1, 01.01.2015, p. 57-63.

Research output: Contribution to journalArticle

Yoshinaga, K, Sasaki, K, Watanabe, H, Nagao, K, Inoue, N, Shirouchi, B, Yanagita, T, Nagai, T, Mizobe, H, Kojima, K, Beppu, F & Gotoh, N 2015, 'Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice', Journal of Nutritional Biochemistry, vol. 26, no. 1, pp. 57-63. https://doi.org/10.1016/j.jnutbio.2014.09.004
Yoshinaga, Kazuaki ; Sasaki, Keiichi ; Watanabe, Hiroyuki ; Nagao, Koji ; Inoue, Nao ; Shirouchi, Bungo ; Yanagita, Teruyoshi ; Nagai, Toshiharu ; Mizobe, Hoyo ; Kojima, Koichi ; Beppu, Fumiaki ; Gotoh, Naohiro. / Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice. In: Journal of Nutritional Biochemistry. 2015 ; Vol. 26, No. 1. pp. 57-63.
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