Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.

Yutaka Kamimura, Keiko Furukawa, Daiji Kittaka, Masashi Nishio, Kazunori Hamamura, Satoshi Fukumoto, Koichi Furukawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

alpha2,8-Sialyltransferase (alpha2,8S-T, GD3 synthase) has been reported to be involved in the enhanced cell proliferation of malignant tumors. Using a cloned cDNA of alpha2,8S-T, transfectant cells were established and the effects of the gene expression on the cell phenotypes were analyzed. In contrast with PC12 cells, in which we reported marked growth enhancement based on the transfection of alpha2,8S-T, Swiss3T3 cells showed no enhancement in either cell proliferation or phosphorylation of MAP kinases after the transfection of alpha2,8S-T when treated with platelet-derived growth factor. Correspondingly, the receptor for platelet-derived growth factor also showed no increased phosphorylation upon the factor stimulation. However, in the wound-healing scratching assay, the Swiss3T3 transfectant cells demonstrated increased mobility as the PC12 transfectant cells. These results suggest that the enhancing effects of alpha2,8S-T on the proliferation and mobility are differential depending on the cell types, and ganglioside-associating molecules in the individual cell types need to be investigated.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalInternational journal of oncology
Volume26
Issue number2
Publication statusPublished - Jan 1 2005
Externally publishedYes

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Sialyltransferases
Cell Proliferation
PC12 Cells
Transfection
Phosphorylation
Platelet-Derived Growth Factor Receptors
Gangliosides
Platelet-Derived Growth Factor
Wound Healing
Phosphotransferases
Complementary DNA
Phenotype
Gene Expression
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kamimura, Y., Furukawa, K., Kittaka, D., Nishio, M., Hamamura, K., Fukumoto, S., & Furukawa, K. (2005). Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility. International journal of oncology, 26(2), 337-344.

Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility. / Kamimura, Yutaka; Furukawa, Keiko; Kittaka, Daiji; Nishio, Masashi; Hamamura, Kazunori; Fukumoto, Satoshi; Furukawa, Koichi.

In: International journal of oncology, Vol. 26, No. 2, 01.01.2005, p. 337-344.

Research output: Contribution to journalArticle

Kamimura, Y, Furukawa, K, Kittaka, D, Nishio, M, Hamamura, K, Fukumoto, S & Furukawa, K 2005, 'Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.', International journal of oncology, vol. 26, no. 2, pp. 337-344.
Kamimura, Yutaka ; Furukawa, Keiko ; Kittaka, Daiji ; Nishio, Masashi ; Hamamura, Kazunori ; Fukumoto, Satoshi ; Furukawa, Koichi. / Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility. In: International journal of oncology. 2005 ; Vol. 26, No. 2. pp. 337-344.
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