Differential expression of cytokeratin after orthotopic implantation of newly established human tongue cancer cell lines of defined metastatic ability

Masayo Morifuji, Shuni’chiro Taniguchi, Hidetaka Sakai, Yusaku Nakabeppu, Masamichi Ohishi

Research output: Contribution to journalArticle

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Abstract

Two human tongue squamous cell carcinoma cell lines, SQUU-A and SQUU-B, were established from the same patient. Cervical lymph node metastasis was detected in the mice orthotopically implanted with SQUU-B (86.7%, 13/15), but not in those with SQUU-A (0/13). Histologically, SQUU-B showed invasive growth and intravasation in the tongue, whereas SQUU-A simply demonstrated expansive growth without intravasation. By Western blot analysis, nonmetastatic clone SQUU-A expressed cytokeratin (CK)13/4, 14, 16/6, 18/8, and 19, whereas a high metastatic clone SQUU-B expressed CK18/8 and 19. The reverse transcription-polymerase chain reaction technique showed that CK13/4 mRNA was expressed in both cell lines, but CK14 and 16 mRNA was expressed only in SQUU-A. CK13 was immunohistochemically expressed in both SQUU-A and SQUU-B transplanted into the tongues of nude mice; CK14 and 16 were detected in SQUU-A of the tongues, but not in SQUU-B. As seen in SQUU-B cell line, SQUU-B of the cervical lymph node metastasis did not exhibit CK13, 14, or 16. These results suggest that the loss or down-regulation of CK13, 14, or 16 is related to the invasive and metastatic ability of cancer. The cytoskeletal system is thus considered to be closely related to the malignant phenotype.

Original languageEnglish
Pages (from-to)1317-1326
Number of pages10
JournalAmerican Journal of Pathology
Volume156
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

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Tongue Neoplasms
Keratins
Tongue
Cell Line
Keratin-4
Keratin-13
Clone Cells
Lymph Nodes
Neoplasm Metastasis
Messenger RNA
Growth
Nude Mice
Reverse Transcription
Squamous Cell Carcinoma
B-Lymphocytes
Down-Regulation
Western Blotting
Phenotype
Polymerase Chain Reaction
Neoplasms

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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Differential expression of cytokeratin after orthotopic implantation of newly established human tongue cancer cell lines of defined metastatic ability. / Morifuji, Masayo; Taniguchi, Shuni’chiro; Sakai, Hidetaka; Nakabeppu, Yusaku; Ohishi, Masamichi.

In: American Journal of Pathology, Vol. 156, No. 4, 01.01.2000, p. 1317-1326.

Research output: Contribution to journalArticle

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abstract = "Two human tongue squamous cell carcinoma cell lines, SQUU-A and SQUU-B, were established from the same patient. Cervical lymph node metastasis was detected in the mice orthotopically implanted with SQUU-B (86.7{\%}, 13/15), but not in those with SQUU-A (0/13). Histologically, SQUU-B showed invasive growth and intravasation in the tongue, whereas SQUU-A simply demonstrated expansive growth without intravasation. By Western blot analysis, nonmetastatic clone SQUU-A expressed cytokeratin (CK)13/4, 14, 16/6, 18/8, and 19, whereas a high metastatic clone SQUU-B expressed CK18/8 and 19. The reverse transcription-polymerase chain reaction technique showed that CK13/4 mRNA was expressed in both cell lines, but CK14 and 16 mRNA was expressed only in SQUU-A. CK13 was immunohistochemically expressed in both SQUU-A and SQUU-B transplanted into the tongues of nude mice; CK14 and 16 were detected in SQUU-A of the tongues, but not in SQUU-B. As seen in SQUU-B cell line, SQUU-B of the cervical lymph node metastasis did not exhibit CK13, 14, or 16. These results suggest that the loss or down-regulation of CK13, 14, or 16 is related to the invasive and metastatic ability of cancer. The cytoskeletal system is thus considered to be closely related to the malignant phenotype.",
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