Differential expression of DNA topoisomerase I gene between CPT-11 acquired-and native-resistant human pancreatic tumor cell lines: Detected by RNA PCR-based quantitation assay

Sachi Takeda, Takao Shimazoe, Keiki Sato, Yoshikazu Sugimoto, Takashi Tsuruo, Akira Kono

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

RNA PCR quantitation method was developed to determine DNA Topoisomerase I(Topo I)-specific mRNA in order to study its gene expression in CPT-11 sensitive, acquired- or native-resistant human pancreatic tumor cell lines. The results were supported by Northern blotting and Western blotting analyses. Acquired-resistant cells have shown decreased levels of Topo I mRNA, compared with their parental cells. On the contrary, in the wild type cells no correlation was shown between sensitivity and gene expression. On the other specific Topo I activity of the native resistant cell lines was fairly lower than that of sensitive cell lines, suggesting that immunoreactive Tope I protein contains low levels of active form enzyme which could be targets of CPT-11 in these native-resistant ones. Finally, the different mechanisms might be operative between acquired- and native-resistant tumor cells.

Original languageEnglish
Pages (from-to)618-625
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number2
DOIs
Publication statusPublished - Apr 30 1992

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irinotecan
Type I DNA Topoisomerase
Tumor Cell Line
Tumors
Assays
Genes
Cells
RNA
Polymerase Chain Reaction
Gene expression
Gene Expression
Cell Line
Messenger RNA
Northern Blotting
Western Blotting
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Differential expression of DNA topoisomerase I gene between CPT-11 acquired-and native-resistant human pancreatic tumor cell lines : Detected by RNA PCR-based quantitation assay. / Takeda, Sachi; Shimazoe, Takao; Sato, Keiki; Sugimoto, Yoshikazu; Tsuruo, Takashi; Kono, Akira.

In: Biochemical and Biophysical Research Communications, Vol. 184, No. 2, 30.04.1992, p. 618-625.

Research output: Contribution to journalArticle

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