Differential expression of major gap junction proteins, connexins 26 and 32, in rat mammary glands during pregnancy and lactation

Ichiro Yamanaka, Akio Kuraoka, Tetsuichiro Inai, Tatsuro Ishibashi, Yosaburo Shibata

Research output: Contribution to journalArticle

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Abstract

We examined the expression pattern of two major gap junction proteins, connexin 26 (Cx26) and connexin 32 (Cx32), in rat mammary glands during pregnancy and lactation. Immunohistochemically the two different Cxs were coexpressed in acinar cells and were independently modulated according to the physiological cell activity. Western blot analysis demonstrated that Cx26 gradually increased from early pregnancy, while Cx32 rapidly and dramatically increased at 16 h after parturition, and that both Cxs reached a maximum early in lactation. Increased expression of both Cxs was confirmed by Northern blot analysis showing that their mRNA transcripts were significantly induced on the day of parturition. We also analyzed double-immunofluorescent staining for Cx26 and Cx32 on a confocal laser scanning microscope, in order to examine colocalization of these Cxs in situ. Cx26 immunoreactivity mostly overlapped with Cx32-positive sites in acinar cells of lactating mammary glands, indicating that both Cxs were colocalized together in the same gap junctional plaques in lactation. These results suggest that upregulation of Cx26 and Cx32 in acinar cells at lactating stages, with colocalization in the same gap junctional plaques, may be important for control of secretion by acinar cells in rat mammary glands.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalHistochemistry and Cell Biology
Volume115
Issue number4
Publication statusPublished - Apr 30 2001

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Connexins
Human Mammary Glands
Lactation
Acinar Cells
Pregnancy
Parturition
Northern Blotting
Lasers
Up-Regulation
Western Blotting
connexin 32
Connexin 26
Staining and Labeling
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Differential expression of major gap junction proteins, connexins 26 and 32, in rat mammary glands during pregnancy and lactation. / Yamanaka, Ichiro; Kuraoka, Akio; Inai, Tetsuichiro; Ishibashi, Tatsuro; Shibata, Yosaburo.

In: Histochemistry and Cell Biology, Vol. 115, No. 4, 30.04.2001, p. 277-284.

Research output: Contribution to journalArticle

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