Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy

Yusuke Sato, Motoyuki Shimizu, Wataru Mizunoya, Hiroyuki Wariishi, Ryuichi Tatsumi, Vladimir L. Buchman, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.

Original languageEnglish
Pages (from-to)1748-1756
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number8
DOIs
Publication statusPublished - Sep 14 2009

Fingerprint

Muscle Denervation
Denervation
Atrophy
Muscle
Rats
Skeletal Muscle
Muscular Atrophy
gamma-Synuclein
Proteins
RNA Editing
Contractile Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Skeletal Muscle Fibers
Enzymes
Proteomics
Gels
Breast Neoplasms
Muscles
Electrophoresis
RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy. / Sato, Yusuke; Shimizu, Motoyuki; Mizunoya, Wataru; Wariishi, Hiroyuki; Tatsumi, Ryuichi; Buchman, Vladimir L.; Ikeuchi, Yoshihide.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 8, 14.09.2009, p. 1748-1756.

Research output: Contribution to journalArticle

Sato, Yusuke ; Shimizu, Motoyuki ; Mizunoya, Wataru ; Wariishi, Hiroyuki ; Tatsumi, Ryuichi ; Buchman, Vladimir L. ; Ikeuchi, Yoshihide. / Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy. In: Bioscience, Biotechnology and Biochemistry. 2009 ; Vol. 73, No. 8. pp. 1748-1756.
@article{596df0d6787d4c009851c33f6a313ba0,
title = "Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy",
abstract = "Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.",
author = "Yusuke Sato and Motoyuki Shimizu and Wataru Mizunoya and Hiroyuki Wariishi and Ryuichi Tatsumi and Buchman, {Vladimir L.} and Yoshihide Ikeuchi",
year = "2009",
month = "9",
day = "14",
doi = "10.1271/bbb.90085",
language = "English",
volume = "73",
pages = "1748--1756",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "8",

}

TY - JOUR

T1 - Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy

AU - Sato, Yusuke

AU - Shimizu, Motoyuki

AU - Mizunoya, Wataru

AU - Wariishi, Hiroyuki

AU - Tatsumi, Ryuichi

AU - Buchman, Vladimir L.

AU - Ikeuchi, Yoshihide

PY - 2009/9/14

Y1 - 2009/9/14

N2 - Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.

AB - Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.

UR - http://www.scopus.com/inward/record.url?scp=69949156922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69949156922&partnerID=8YFLogxK

U2 - 10.1271/bbb.90085

DO - 10.1271/bbb.90085

M3 - Article

C2 - 19661702

AN - SCOPUS:69949156922

VL - 73

SP - 1748

EP - 1756

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 8

ER -