Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease

S. Chen, Y. Moroi, K. Urabe, S. Takeuchi, Makiko Nakahara, S. Hayashida, Uchi Hiroshi, T. Uenotsuchi, Y. Tu, Masutaka Furue

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Abstract

Background. The proteins p53, p63 and p73 are known to be overexpressed and to play important roles in the pathogenesis of many tumours, but the expression of p63 and p73 has not previously been investigated in extramammary Paget's disease (EMPD). Aim. To investigate the potential contribution of p53, p63 and p73 in the pathogenesis of EMPD. Methods. In total, 35 paraffin wax-embedded tissue samples from patients with EMPD were examined using immunohistochemical staining for p53, p63 and p73. Results. All of the 35 EMPD specimens, including all 6 invasive EMPD and 2 metastatic lymph-node specimens, showed nuclear overexpression of both p53 and p73. The expression levels (percentage of positive cells) of p53 and p73 (90.66 ± 12.53% and 80.20 ± 13.07%) in EMPD were significantly higher than those of normal skin. There was a significant correlation between the expression levels of p53 and p73 in EMPD. In 29 of 35 EMPD specimens, there was no nuclear expression of p63, and weak or moderate staining was found in only 6 specimens. The expression level of p63 in EMPD was significantly less than that in normal skin. Conclusions. Our study shows that the concordant overexpression of p53 and p73 and the decreased expression of p63 may play a pivotal role in the pathogenesis of EMPD. The decreased expression of p63 may play a more important role in the pathogenesis of EMPD than the overexpression of p53 and p73.

Original languageEnglish
Pages (from-to)634-640
Number of pages7
JournalClinical and Experimental Dermatology
Volume33
Issue number5
DOIs
Publication statusPublished - Sep 1 2008

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Extramammary Paget's Disease
Staining and Labeling
Skin
Waxes
Paraffin

All Science Journal Classification (ASJC) codes

  • Dermatology

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Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease. / Chen, S.; Moroi, Y.; Urabe, K.; Takeuchi, S.; Nakahara, Makiko; Hayashida, S.; Hiroshi, Uchi; Uenotsuchi, T.; Tu, Y.; Furue, Masutaka.

In: Clinical and Experimental Dermatology, Vol. 33, No. 5, 01.09.2008, p. 634-640.

Research output: Contribution to journalArticle

Chen, S. ; Moroi, Y. ; Urabe, K. ; Takeuchi, S. ; Nakahara, Makiko ; Hayashida, S. ; Hiroshi, Uchi ; Uenotsuchi, T. ; Tu, Y. ; Furue, Masutaka. / Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease. In: Clinical and Experimental Dermatology. 2008 ; Vol. 33, No. 5. pp. 634-640.
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abstract = "Background. The proteins p53, p63 and p73 are known to be overexpressed and to play important roles in the pathogenesis of many tumours, but the expression of p63 and p73 has not previously been investigated in extramammary Paget's disease (EMPD). Aim. To investigate the potential contribution of p53, p63 and p73 in the pathogenesis of EMPD. Methods. In total, 35 paraffin wax-embedded tissue samples from patients with EMPD were examined using immunohistochemical staining for p53, p63 and p73. Results. All of the 35 EMPD specimens, including all 6 invasive EMPD and 2 metastatic lymph-node specimens, showed nuclear overexpression of both p53 and p73. The expression levels (percentage of positive cells) of p53 and p73 (90.66 ± 12.53{\%} and 80.20 ± 13.07{\%}) in EMPD were significantly higher than those of normal skin. There was a significant correlation between the expression levels of p53 and p73 in EMPD. In 29 of 35 EMPD specimens, there was no nuclear expression of p63, and weak or moderate staining was found in only 6 specimens. The expression level of p63 in EMPD was significantly less than that in normal skin. Conclusions. Our study shows that the concordant overexpression of p53 and p73 and the decreased expression of p63 may play a pivotal role in the pathogenesis of EMPD. The decreased expression of p63 may play a more important role in the pathogenesis of EMPD than the overexpression of p53 and p73.",
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T1 - Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease

AU - Chen, S.

AU - Moroi, Y.

AU - Urabe, K.

AU - Takeuchi, S.

AU - Nakahara, Makiko

AU - Hayashida, S.

AU - Hiroshi, Uchi

AU - Uenotsuchi, T.

AU - Tu, Y.

AU - Furue, Masutaka

PY - 2008/9/1

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N2 - Background. The proteins p53, p63 and p73 are known to be overexpressed and to play important roles in the pathogenesis of many tumours, but the expression of p63 and p73 has not previously been investigated in extramammary Paget's disease (EMPD). Aim. To investigate the potential contribution of p53, p63 and p73 in the pathogenesis of EMPD. Methods. In total, 35 paraffin wax-embedded tissue samples from patients with EMPD were examined using immunohistochemical staining for p53, p63 and p73. Results. All of the 35 EMPD specimens, including all 6 invasive EMPD and 2 metastatic lymph-node specimens, showed nuclear overexpression of both p53 and p73. The expression levels (percentage of positive cells) of p53 and p73 (90.66 ± 12.53% and 80.20 ± 13.07%) in EMPD were significantly higher than those of normal skin. There was a significant correlation between the expression levels of p53 and p73 in EMPD. In 29 of 35 EMPD specimens, there was no nuclear expression of p63, and weak or moderate staining was found in only 6 specimens. The expression level of p63 in EMPD was significantly less than that in normal skin. Conclusions. Our study shows that the concordant overexpression of p53 and p73 and the decreased expression of p63 may play a pivotal role in the pathogenesis of EMPD. The decreased expression of p63 may play a more important role in the pathogenesis of EMPD than the overexpression of p53 and p73.

AB - Background. The proteins p53, p63 and p73 are known to be overexpressed and to play important roles in the pathogenesis of many tumours, but the expression of p63 and p73 has not previously been investigated in extramammary Paget's disease (EMPD). Aim. To investigate the potential contribution of p53, p63 and p73 in the pathogenesis of EMPD. Methods. In total, 35 paraffin wax-embedded tissue samples from patients with EMPD were examined using immunohistochemical staining for p53, p63 and p73. Results. All of the 35 EMPD specimens, including all 6 invasive EMPD and 2 metastatic lymph-node specimens, showed nuclear overexpression of both p53 and p73. The expression levels (percentage of positive cells) of p53 and p73 (90.66 ± 12.53% and 80.20 ± 13.07%) in EMPD were significantly higher than those of normal skin. There was a significant correlation between the expression levels of p53 and p73 in EMPD. In 29 of 35 EMPD specimens, there was no nuclear expression of p63, and weak or moderate staining was found in only 6 specimens. The expression level of p63 in EMPD was significantly less than that in normal skin. Conclusions. Our study shows that the concordant overexpression of p53 and p73 and the decreased expression of p63 may play a pivotal role in the pathogenesis of EMPD. The decreased expression of p63 may play a more important role in the pathogenesis of EMPD than the overexpression of p53 and p73.

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