Differential expression sites of TGF-β isoforms in chicken limb buds during morphogenesis

Shinya Aramaki, Fuminori Sato, Tomoki Soh, Nobuhiko Yamauchi, Masa Aki Hattori

Research output: Contribution to journalArticle

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Abstract

TGF-β gene is expressed at various developmental stages and its principle role may be an involvement in organogenesis. The present study was performed to investigate the temporal expression of these TGF-β isoforms in the developing limb of White Leghorn Chicken, Gallus gallus (L., 1758). TGF-β isoforms were expressed in the developing limb as revealed by whole-mount in situ hybridization, but each showed a different pattern of expression. TGF-β2 was the dominant isoform compared with the other two isoforms. TGF-β2 first appeared along the proximodistal axis of the limb at stage 24 and condensed at the tip at stage 26. At stages 29-31, expression appeared in digits and then was extended to the interdigital spaces. A weak signal for TGF-β3 was first shown in the developing limb at stage 26, but there was no interdigital expression, unlike for TGF-β2. TGF-β4 was expressed in the developing limb at stage 26 and only in the interdigital spaces at stage 29. Reverse transcription - polymerase chain reaction analysis also showed that the transcript levels of TGF-β isoforms, especially TGF-β2, drastically increased at stage 29. These results suggest that TGF-β isoforms, with their patterns of expression, are specific regulatory factors that participate in limb development and digit morphogenesis.

Original languageEnglish
Pages (from-to)620-625
Number of pages6
JournalCanadian Journal of Zoology
Volume83
Issue number4
DOIs
Publication statusPublished - Apr 2005

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limb bud
morphogenesis
limbs (animal)
bud
limb
chickens
Gallus gallus
White Leghorn
organogenesis
developmental stage
in situ hybridization
polymerase chain reaction
reverse transcriptase polymerase chain reaction
developmental stages
gene

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Differential expression sites of TGF-β isoforms in chicken limb buds during morphogenesis. / Aramaki, Shinya; Sato, Fuminori; Soh, Tomoki; Yamauchi, Nobuhiko; Hattori, Masa Aki.

In: Canadian Journal of Zoology, Vol. 83, No. 4, 04.2005, p. 620-625.

Research output: Contribution to journalArticle

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