TY - JOUR
T1 - Differential gene dosage effects of Ad4BP/SF-1 on target tissue development
AU - Fatchiyah,
AU - Zubair, Mohamad
AU - Shima, Yuichi
AU - Oka, Sanae
AU - Ishihara, Satoru
AU - Fukui-Katoh, Yuko
AU - Morohashi, Ken Ichirou
N1 - Funding Information:
We thank Drs. X.W. Yang and N. Heintz (Rockefeller University) for providing the homologous recombination system of BAC clone and pSV1 RecA, and Drs. K. Mihara and M. Sakaguchi (Kyushu University) for providing antiserum to lacZ. We also thank Technological and Professionalism Skill for Developmental Project of SPMU Brawijaya University and CPMU-DGHE of The Ministry of National Education, Indonesia. This work was supported in part by Grants-in-Aid for Scientific Research (S) and Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2006/3/24
Y1 - 2006/3/24
N2 - Ad4BP/SF-1 (NR5A1) was identified as a key regulator of the hypothalamus-pituitary-gonadal and -adrenal axes. Loss-of-function studies revealed that Ad4BP/SF-1 is essential for the development of these tissues and spleen. Here, we generated transgenic mouse with BAC recombinants carrying a dual promoter and Tet-off system. These recombinants have a potential to express lacZ and Ad4BP/SF-1 in the tissues where endogenous Ad4BP/SF-1 is expressed. However, protein level of Ad4BP/SF-1 varied among the tissues of the transgenic mice and probably thereby the target tissues are affected differentially. The BAC-transgenic mice were applied to rescue Ad4BP/SF-1 KO mouse. Interestingly, the mice successfully rescued the gonad and spleen but failed to rescue the adrenal gland. This variation might be dependent on in part the protein expression levels among the tissues and in part on differential sensitivities to the gene dosage.
AB - Ad4BP/SF-1 (NR5A1) was identified as a key regulator of the hypothalamus-pituitary-gonadal and -adrenal axes. Loss-of-function studies revealed that Ad4BP/SF-1 is essential for the development of these tissues and spleen. Here, we generated transgenic mouse with BAC recombinants carrying a dual promoter and Tet-off system. These recombinants have a potential to express lacZ and Ad4BP/SF-1 in the tissues where endogenous Ad4BP/SF-1 is expressed. However, protein level of Ad4BP/SF-1 varied among the tissues of the transgenic mice and probably thereby the target tissues are affected differentially. The BAC-transgenic mice were applied to rescue Ad4BP/SF-1 KO mouse. Interestingly, the mice successfully rescued the gonad and spleen but failed to rescue the adrenal gland. This variation might be dependent on in part the protein expression levels among the tissues and in part on differential sensitivities to the gene dosage.
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U2 - 10.1016/j.bbrc.2006.01.058
DO - 10.1016/j.bbrc.2006.01.058
M3 - Article
C2 - 16458255
AN - SCOPUS:32344440660
SN - 0006-291X
VL - 341
SP - 1036
EP - 1045
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -