Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons

Mihoko Inamura, Makoto Itakura, Hirotsugu Okamoto, Sumio Hoka, Akira Mizoguchi, Yugo Fukazawa, Ryuichi Shigemoto, Saori Yamamori, Masami Takahashi

Research output: Contribution to journalArticle

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Abstract

Transmembrane AMPA receptor regulatory proteins (TARPs), including stargazin/γ-2, are associated with AMPA receptors and participate in their surface delivery and anchoring at the postsynaptic membrane. TARPs may also act as a positive modulator of the AMPA receptor ion channel function; however, little is known about other TARP members except for stargazin/γ-2. We examined the synaptic localization of stargazin/γ-2 and γ-8 by immunoelectron microscopy and biochemical analysis. The analysis of sodium dodecyl sulfate-digested freeze-fracture replica labeling revealed that stargazin/γ-2 was concentrated in the postsynaptic area, whereas γ-8 was distributed both in synaptic and extra-synaptic plasma membranes of the hippocampal neuron. When a synaptic plasma membrane-enriched brain fraction was treated with Triton X-100 and separated by sucrose density gradient ultracentrifugation, a large proportion of NMDA receptor and stargazin/γ-2 was accumulated in raft-enriched fractions, whereas AMPA receptor and γ-8 were distributed in both the raft-enriched fractions and other Triton-insoluble fractions. Phosphorylation of stargazin/γ-2 and γ-8 was regulated by different sets of kinases and phosphatases in cultured cortical neurons. These results suggested that stargazin/γ-2 and γ-8 have distinct roles in postsynaptic membranes under the regulation of different intracellular signaling pathways.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalNeuroscience Research
Volume55
Issue number1
DOIs
Publication statusPublished - May 1 2006

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AMPA Receptors
Cell Membrane
Neurons
Synaptic Membranes
Proteins
Membranes
Immunoelectron Microscopy
Ultracentrifugation
Octoxynol
N-Methyl-D-Aspartate Receptors
Ion Channels
Phosphoric Monoester Hydrolases
Sodium Dodecyl Sulfate
Sucrose
Phosphotransferases
Phosphorylation
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. / Inamura, Mihoko; Itakura, Makoto; Okamoto, Hirotsugu; Hoka, Sumio; Mizoguchi, Akira; Fukazawa, Yugo; Shigemoto, Ryuichi; Yamamori, Saori; Takahashi, Masami.

In: Neuroscience Research, Vol. 55, No. 1, 01.05.2006, p. 45-53.

Research output: Contribution to journalArticle

Inamura, M, Itakura, M, Okamoto, H, Hoka, S, Mizoguchi, A, Fukazawa, Y, Shigemoto, R, Yamamori, S & Takahashi, M 2006, 'Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons', Neuroscience Research, vol. 55, no. 1, pp. 45-53. https://doi.org/10.1016/j.neures.2006.01.004
Inamura, Mihoko ; Itakura, Makoto ; Okamoto, Hirotsugu ; Hoka, Sumio ; Mizoguchi, Akira ; Fukazawa, Yugo ; Shigemoto, Ryuichi ; Yamamori, Saori ; Takahashi, Masami. / Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. In: Neuroscience Research. 2006 ; Vol. 55, No. 1. pp. 45-53.
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