Abstract
Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.
| Original language | English |
|---|---|
| Pages (from-to) | 175-180 |
| Number of pages | 6 |
| Journal | Histochemistry and Cell Biology |
| Volume | 114 |
| Issue number | 3 |
| Publication status | Published - Oct 23 2000 |
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All Science Journal Classification (ASJC) codes
- Histology
- Molecular Biology
- Medical Laboratory Technology
- Cell Biology
Cite this
Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules. / Sugawara-Yokoo, Minako; Urakami, Yumiko; Koyama, Haruko; Fujikura, Keiko; Masuda, Satohiro; Saito, Hideyuki; Naruse, Takuji; Inui, Ken Ichi; Takata, Kuniaki.
In: Histochemistry and Cell Biology, Vol. 114, No. 3, 23.10.2000, p. 175-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules
AU - Sugawara-Yokoo, Minako
AU - Urakami, Yumiko
AU - Koyama, Haruko
AU - Fujikura, Keiko
AU - Masuda, Satohiro
AU - Saito, Hideyuki
AU - Naruse, Takuji
AU - Inui, Ken Ichi
AU - Takata, Kuniaki
PY - 2000/10/23
Y1 - 2000/10/23
N2 - Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.
AB - Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033770499&partnerID=8YFLogxK
M3 - Article
C2 - 11083459
AN - SCOPUS:0033770499
VL - 114
SP - 175
EP - 180
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 3
ER -