Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules

Minako Sugawara-Yokoo, Yumiko Urakami, Haruko Koyama, Keiko Fujikura, Satohiro Masuda, Hideyuki Saito, Takuji Naruse, Ken Ichi Inui, Kuniaki Takata

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Abstract

Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalHistochemistry and Cell Biology
Volume114
Issue number3
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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    Sugawara-Yokoo, M., Urakami, Y., Koyama, H., Fujikura, K., Masuda, S., Saito, H., Naruse, T., Inui, K. I., & Takata, K. (2000). Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules. Histochemistry and Cell Biology, 114(3), 175-180. https://doi.org/10.1007/s004180000186