Differential regulation of cellular maturation in chondrocytes and osteoblasts by glycine

Yoshifumi Takahata, Takeshi Takarada, Masato Osawa, Eiichi Hinoi, Yukari Kyumoto, Yukio Yoneda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previous studies have demonstrated the functional expression, by osteoblasts, of N-methyl-D-aspartate (NMDA) receptors responsible for the promotion of cellular differentiation in bone. We have now evaluated the possible role of the endogenous co-agonist of NMDA receptors, glycine (Gly), in chondrogenesis. In ex vivo organotypic cultures of fetal mouse tibias, proximal and distal cartilaginous primordia were significantly increased in the presence of Gly, with the osteogenic center being unchanged. Exposure to Gly drastically increased mRNA expression of the calcified chondrocyte marker osteopontin, without markedly affecting that of a proliferating chondrocyte marker or a hypertrophic chondrocyte marker, as shown in organotypic cultures by in situ hybridization analysis. Gly significantly increased Ca2+ accumulation, osteopontin mRNA expression, and alkaline phosphatase activity in cultured rat costal chondrocytes, without significantly affecting those in cultured rat calvarial osteoblasts. The increase induced by Gly was significantly prevented by an NMDA receptor channel blocker and an antagonist at the Gly site on NMDA receptors, but not by an inhibitory Gly receptor antagonist or a Gly transporter inhibitor, in cultured chondrocytes. Constitutive mRNA expression was seen for NR1, NR2D, and NR3A subunits of NMDA receptors, but not for Gly receptors and transporters, in cultured chondrocytes. Corresponding immunoreactive proteins were detected for NR1 and NR2D subunits in cartilaginous zones of fetal mouse tibias. Thus, Gly might, at least in part, play a role as a trophic factor in the mechanisms associated with chondral calcification through the Gly site of NMDA receptors functionally expressed by chondrocytes in rodent cartilage.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalCell and tissue research
Volume333
Issue number1
DOIs
Publication statusPublished - Jul 1 2008
Externally publishedYes

Fingerprint

Osteoblasts
Chondrocytes
Glycine
N-Methyl-D-Aspartate Receptors
Glycine Plasma Membrane Transport Proteins
Glycine Receptors
Osteopontin
Tibia
Messenger RNA
Cartilage
Rats
Chondrogenesis
In Situ Hybridization
Alkaline Phosphatase
Rodentia
Bone
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Differential regulation of cellular maturation in chondrocytes and osteoblasts by glycine. / Takahata, Yoshifumi; Takarada, Takeshi; Osawa, Masato; Hinoi, Eiichi; Kyumoto, Yukari; Yoneda, Yukio.

In: Cell and tissue research, Vol. 333, No. 1, 01.07.2008, p. 91-103.

Research output: Contribution to journalArticle

Takahata, Yoshifumi ; Takarada, Takeshi ; Osawa, Masato ; Hinoi, Eiichi ; Kyumoto, Yukari ; Yoneda, Yukio. / Differential regulation of cellular maturation in chondrocytes and osteoblasts by glycine. In: Cell and tissue research. 2008 ; Vol. 333, No. 1. pp. 91-103.
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