Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Yutaka Okuno, Hiromi Iwasaki, Claudia S. Huettner, Hanna S. Radomska, David A. Gonzalez, Daniel G. Tenen, Koichi Akashi

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34-/lo express the hCD34 transgene. These data strongly support the notion that hCD34+ human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

Original languageEnglish
Pages (from-to)6246-6251
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number9
DOIs
Publication statusPublished - Apr 30 2002
Externally publishedYes

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Hematopoietic Stem Cells
Genes
Hematopoiesis
Transgenic Mice
Artificial Chromosomes
Transgenes
Bone Marrow Cells
Clone Cells
Transplantation
Gene Expression
Population

All Science Journal Classification (ASJC) codes

  • General

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Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. / Okuno, Yutaka; Iwasaki, Hiromi; Huettner, Claudia S.; Radomska, Hanna S.; Gonzalez, David A.; Tenen, Daniel G.; Akashi, Koichi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 9, 30.04.2002, p. 6246-6251.

Research output: Contribution to journalArticle

Okuno, Yutaka ; Iwasaki, Hiromi ; Huettner, Claudia S. ; Radomska, Hanna S. ; Gonzalez, David A. ; Tenen, Daniel G. ; Akashi, Koichi. / Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 9. pp. 6246-6251.
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