TY - JOUR
T1 - Differential regulation of thymus- and activation-regulated chemokine induced by IL-4, IL-13, TNF-α and IFN-γ in human keratinocyte and fibroblast
AU - Yu, Bin
AU - Koga, Tetsuya
AU - Urabe, Kazunori
AU - Moroi, Yoichi
AU - Maeda, Shoko
AU - Yanagihara, Yukiyoshi
AU - Furue, Masutaka
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan, and also from the Ministry of Health and Welfare of Japan. We thank Dr Naoko Kinnkawa very much for her suggestion for statistical analysis.
PY - 2002/10
Y1 - 2002/10
N2 - The CC chemokine thymus- and activation-regulated chemokine (TARC/CCL17) acts on CC chemokine receptor 4 (CCR4), which is known to be selectively expressed in Th2 cells. In order to compare the regulatory profiles of TARC production by tumor necrosis factor-α (TNF-α), IFN-γ, interleukin-4 (IL-4) and IL-13 in keratinocytes and fibroblasts, HaCaT cells, a human keratinocyte cell line, and NG1RGB cells, a human skin fibroblast cell line, were used. The expression of TARC protein was measured using enzyme-linked immunosorbent assay (ELISA), and the mRNA level was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The spontaneous expression of TARC protein and mRNA levels were augmented by TNF-α and IFN-γ and were inhibited by IL-4 and IL-13 in the keratinocytes. The fibroblasts expressed the TARC protein and mRNA only in the presence of IL-4+TNF-α or IL-13+TNF-α stimulation. IFN-γ further enhanced the IL-4+TNF-α or IL-13+TNF-α-induced TARC production in the fibroblasts. Thus, TNF-α and IFN-γ -induced TARC production was differentially regulated by IL-4 and IL-13 in human keratinocytes and fibroblasts.
AB - The CC chemokine thymus- and activation-regulated chemokine (TARC/CCL17) acts on CC chemokine receptor 4 (CCR4), which is known to be selectively expressed in Th2 cells. In order to compare the regulatory profiles of TARC production by tumor necrosis factor-α (TNF-α), IFN-γ, interleukin-4 (IL-4) and IL-13 in keratinocytes and fibroblasts, HaCaT cells, a human keratinocyte cell line, and NG1RGB cells, a human skin fibroblast cell line, were used. The expression of TARC protein was measured using enzyme-linked immunosorbent assay (ELISA), and the mRNA level was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The spontaneous expression of TARC protein and mRNA levels were augmented by TNF-α and IFN-γ and were inhibited by IL-4 and IL-13 in the keratinocytes. The fibroblasts expressed the TARC protein and mRNA only in the presence of IL-4+TNF-α or IL-13+TNF-α stimulation. IFN-γ further enhanced the IL-4+TNF-α or IL-13+TNF-α-induced TARC production in the fibroblasts. Thus, TNF-α and IFN-γ -induced TARC production was differentially regulated by IL-4 and IL-13 in human keratinocytes and fibroblasts.
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U2 - 10.1016/S0923-1811(02)00046-4
DO - 10.1016/S0923-1811(02)00046-4
M3 - Article
C2 - 12354417
AN - SCOPUS:0036804493
SN - 0923-1811
VL - 30
SP - 29
EP - 36
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
IS - 1
ER -