Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation

Tadafumi Asaoka, Tomoaki Kato, Shigeru Marubashi, Keizo Dono, Naoki Hama, Hidenori Takahashi, Shogo Kobayashi, Yutaka Takeda, Ichiro Takemasa, Hiroaki Nagano, Hideo Yoshida, Phillip Ruiz, Andreas G. Tzakis, Kenichi Matsubara, Morito Monden, Yuichiro Doki, Masaki Mori

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Histopathological evaluation of the liver via biopsy remains the standard procedure for the diagnosis of both acute cellular rejection (ACR) and recurrent hepatitis C (RHC) after liver transplantation. Nevertheless, it is often difficult to diagnose ACR in hepatitis C virus-positive recipients because of changes in common and overlapping with RHC. The aim of this study was to identify potential target genes for ACR in recipients with RHC. We analyzed 22 liver biopsy samples obtained from 21 hepatitis C virus-positive recipients. The clinicopathological diagnosis based on biopsy examination was ACR-predominant with superimposed RHC in 9 samples (ACR group) and RHC without ACR (non-ACR group) in 13. Using oligonucleotide microarrays, we compared the transcriptional changes in the 2 groups and selected 2206 genes that were significantly modulated in ACR. We analyzed the regulatory networks in ACR with Ingenuity Pathway Analysis software, and we confirmed with quantitative real-time polymerase chain reaction the reproducibility of caspase 8, apoptosis-related cysteine peptidase and bone morphogenetic protein 2 up-regulation in another group of validation samples, representing 2 genes from the core network as the target genes for ACR. Our results demonstrated novel transcriptome patterns for ACR with concurrent RHC that were distinct from those of recipients with only RHC, suggesting that gene expression profiling may be useful in the diagnosis of ACR in recipients with hepatitis C.

Original languageEnglish
Pages (from-to)1738-1749
Number of pages12
JournalLiver Transplantation
Volume15
Issue number12
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

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Hepatitis C
Transcriptome
Liver Transplantation
Biopsy
Hepacivirus
Genes
Bone Morphogenetic Protein 2
Caspase 8
Gene Regulatory Networks
Liver
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Cysteine
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Up-Regulation
Software
Apoptosis

All Science Journal Classification (ASJC) codes

  • Surgery
  • Hepatology
  • Transplantation

Cite this

Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation. / Asaoka, Tadafumi; Kato, Tomoaki; Marubashi, Shigeru; Dono, Keizo; Hama, Naoki; Takahashi, Hidenori; Kobayashi, Shogo; Takeda, Yutaka; Takemasa, Ichiro; Nagano, Hiroaki; Yoshida, Hideo; Ruiz, Phillip; Tzakis, Andreas G.; Matsubara, Kenichi; Monden, Morito; Doki, Yuichiro; Mori, Masaki.

In: Liver Transplantation, Vol. 15, No. 12, 01.12.2009, p. 1738-1749.

Research output: Contribution to journalArticle

Asaoka, T, Kato, T, Marubashi, S, Dono, K, Hama, N, Takahashi, H, Kobayashi, S, Takeda, Y, Takemasa, I, Nagano, H, Yoshida, H, Ruiz, P, Tzakis, AG, Matsubara, K, Monden, M, Doki, Y & Mori, M 2009, 'Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation', Liver Transplantation, vol. 15, no. 12, pp. 1738-1749. https://doi.org/10.1002/lt.21883
Asaoka, Tadafumi ; Kato, Tomoaki ; Marubashi, Shigeru ; Dono, Keizo ; Hama, Naoki ; Takahashi, Hidenori ; Kobayashi, Shogo ; Takeda, Yutaka ; Takemasa, Ichiro ; Nagano, Hiroaki ; Yoshida, Hideo ; Ruiz, Phillip ; Tzakis, Andreas G. ; Matsubara, Kenichi ; Monden, Morito ; Doki, Yuichiro ; Mori, Masaki. / Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation. In: Liver Transplantation. 2009 ; Vol. 15, No. 12. pp. 1738-1749.
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