Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N-nitrosoguanidine exposure

Chie Furihata, Masashi Oka, Masami Yamamoto, Takashi Ito, Masao Ichinose, Kazumasa Miki, Masae Tatematsu, Yoshiyuki Sakaki, Konrad Reske

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Administration of N-methyl-N'-nitro-N-nitrosoguanidine, a glandular stomach carcinogen, at the concentration of 100 μg/ml in drinking water for 8 days induced the appearance of a MHC class II-associated invariant chain in the target organ of stomach pyloric mucosa of male Lewis rats. The up- regulation of the MHC class II-associated invariant chain was revealed by fluorescent differential display analysis, reverse transcription-PCR, Northern blot, and histochemical staining. The appearance of MHC class II and MHC class I was also demonstrated by reverse transcription-PCR and Northern blot. The results suggest the involvement of MHC-controlled immune reactions in chemically-induced stomach carcinogenesis.

Original languageEnglish
Pages (from-to)1416-1418
Number of pages3
JournalCancer Research
Volume57
Issue number8
Publication statusPublished - May 6 1997
Externally publishedYes

Fingerprint

Methylnitronitrosoguanidine
Stomach
Mucous Membrane
Northern Blotting
Reverse Transcription
Polymerase Chain Reaction
Drinking Water
Carcinogens
Carcinogenesis
Up-Regulation
Staining and Labeling
invariant chain

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N-nitrosoguanidine exposure. / Furihata, Chie; Oka, Masashi; Yamamoto, Masami; Ito, Takashi; Ichinose, Masao; Miki, Kazumasa; Tatematsu, Masae; Sakaki, Yoshiyuki; Reske, Konrad.

In: Cancer Research, Vol. 57, No. 8, 06.05.1997, p. 1416-1418.

Research output: Contribution to journalArticle

Furihata, C, Oka, M, Yamamoto, M, Ito, T, Ichinose, M, Miki, K, Tatematsu, M, Sakaki, Y & Reske, K 1997, 'Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N-nitrosoguanidine exposure', Cancer Research, vol. 57, no. 8, pp. 1416-1418.
Furihata, Chie ; Oka, Masashi ; Yamamoto, Masami ; Ito, Takashi ; Ichinose, Masao ; Miki, Kazumasa ; Tatematsu, Masae ; Sakaki, Yoshiyuki ; Reske, Konrad. / Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N-nitrosoguanidine exposure. In: Cancer Research. 1997 ; Vol. 57, No. 8. pp. 1416-1418.
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