Differentiation of induced pluripotent stem cells into dental mesenchymal cells

Keishi Otsu, Ryota Kishigami, Ai Oikawa-Sasaki, Satoshi Fukumoto, Aya Yamada, Naoki Fujiwara, Kiyoto Ishizeki, Hidemitsu Harada

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Similar to embryonic stem cells, induced pluripotent stem (iPS) cells can differentiate into various cell types upon appropriate induction, and thus, may be valuable cell sources for regenerative medicine. However, iPS cells have not been reported to differentiate into odontogenic cells for tooth regeneration. Here we demonstrated that neural crest-like cells (NCLC) derived from mouse iPS cells have the potential to differentiate into odontogenic mesenchymal cells. We developed an efficient culture protocol to induce the differentiation of mouse iPS cells into NCLC. We confirmed that the cells exhibited neural crest (NC) cell markers as evidenced by immunocytochemistry, flow cytometry, and real-time reverse transcription-polymerase chain reaction. Further, in recombination cultures of NCLC and mouse dental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.

Original languageEnglish
Pages (from-to)1156-1164
Number of pages9
JournalStem cells and development
Volume21
Issue number7
DOIs
Publication statusPublished - May 1 2012
Externally publishedYes

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Induced Pluripotent Stem Cells
Neural Crest
Tooth
Regeneration
Epithelium
Odontoblasts
Regenerative Medicine
Embryonic Stem Cells
Conditioned Culture Medium
Genetic Recombination
Reverse Transcription
Flow Cytometry
Immunohistochemistry
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Otsu, K., Kishigami, R., Oikawa-Sasaki, A., Fukumoto, S., Yamada, A., Fujiwara, N., ... Harada, H. (2012). Differentiation of induced pluripotent stem cells into dental mesenchymal cells. Stem cells and development, 21(7), 1156-1164. https://doi.org/10.1089/scd.2011.0210

Differentiation of induced pluripotent stem cells into dental mesenchymal cells. / Otsu, Keishi; Kishigami, Ryota; Oikawa-Sasaki, Ai; Fukumoto, Satoshi; Yamada, Aya; Fujiwara, Naoki; Ishizeki, Kiyoto; Harada, Hidemitsu.

In: Stem cells and development, Vol. 21, No. 7, 01.05.2012, p. 1156-1164.

Research output: Contribution to journalArticle

Otsu, K, Kishigami, R, Oikawa-Sasaki, A, Fukumoto, S, Yamada, A, Fujiwara, N, Ishizeki, K & Harada, H 2012, 'Differentiation of induced pluripotent stem cells into dental mesenchymal cells', Stem cells and development, vol. 21, no. 7, pp. 1156-1164. https://doi.org/10.1089/scd.2011.0210
Otsu, Keishi ; Kishigami, Ryota ; Oikawa-Sasaki, Ai ; Fukumoto, Satoshi ; Yamada, Aya ; Fujiwara, Naoki ; Ishizeki, Kiyoto ; Harada, Hidemitsu. / Differentiation of induced pluripotent stem cells into dental mesenchymal cells. In: Stem cells and development. 2012 ; Vol. 21, No. 7. pp. 1156-1164.
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