Differentiation of Lens and Pigment Cells in Cultures of Brain Cells of Chick Embryos

KAZUYA NOMURA

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Dissociated cells of brains (tel- and diencephalons) of 3.5-day-old chick embryos were cultivated in vitro under the cell culture conditions which are known to be permissive for neural retinal cells (NR cells) to transdifferentiate into lens and/or pigmented epithelial cells (PE cells). The differentiation of lentoid bodies (LBs) with lens-specific (δ-crystallin and PE cells with melanin granules was observed in such brain cultures. LBs appeared in two different phases, i. e., 2–3 days and 16–30 days of cultivation, and after 40 days of culture these structures were formed in all 60 culture dishes. Sometimes, LBs were observed in foci of PE cells formed during earlier stages of brain cultures. When similar brain cultures were prepared with older embryos of 5-, 8.5-, 14-, and 16-days of incubation, no differentiation of lens and PE cells was observed.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalDifferentiation
Volume22
Issue number1-3
DOIs
Publication statusPublished - Jan 1 1982

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Chick Embryo
Lenses
Cell Culture Techniques
Epithelial Cells
Brain
Diencephalon
Telencephalon
Crystallins
Melanins
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Differentiation of Lens and Pigment Cells in Cultures of Brain Cells of Chick Embryos. / NOMURA, KAZUYA.

In: Differentiation, Vol. 22, No. 1-3, 01.01.1982, p. 179-184.

Research output: Contribution to journalArticle

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