TY - JOUR
T1 - Differentiation of Lens and Pigment Cells in Cultures of Brain Cells of Chick Embryos
AU - NOMURA, KAZUYA
N1 - Funding Information:
Acknowledgements. The author wishes to express his sincerest gratitude to Professor T. S. Okada for his advice and critical reading of the manuscript, and to thank Drs. M. Takeichi, K. Yasuda, and H. Kondoh for their advice. The present work was supported in part by a research grant for basic cancer research from the Japanese Ministry of Education, Science, and Culture and a research grant from the Yamada Foundation to Professor T. S. Okada.
PY - 1982
Y1 - 1982
N2 - Dissociated cells of brains (tel- and diencephalons) of 3.5-day-old chick embryos were cultivated in vitro under the cell culture conditions which are known to be permissive for neural retinal cells (NR cells) to transdifferentiate into lens and/or pigmented epithelial cells (PE cells). The differentiation of lentoid bodies (LBs) with lens-specific (δ-crystallin and PE cells with melanin granules was observed in such brain cultures. LBs appeared in two different phases, i. e., 2–3 days and 16–30 days of cultivation, and after 40 days of culture these structures were formed in all 60 culture dishes. Sometimes, LBs were observed in foci of PE cells formed during earlier stages of brain cultures. When similar brain cultures were prepared with older embryos of 5-, 8.5-, 14-, and 16-days of incubation, no differentiation of lens and PE cells was observed.
AB - Dissociated cells of brains (tel- and diencephalons) of 3.5-day-old chick embryos were cultivated in vitro under the cell culture conditions which are known to be permissive for neural retinal cells (NR cells) to transdifferentiate into lens and/or pigmented epithelial cells (PE cells). The differentiation of lentoid bodies (LBs) with lens-specific (δ-crystallin and PE cells with melanin granules was observed in such brain cultures. LBs appeared in two different phases, i. e., 2–3 days and 16–30 days of cultivation, and after 40 days of culture these structures were formed in all 60 culture dishes. Sometimes, LBs were observed in foci of PE cells formed during earlier stages of brain cultures. When similar brain cultures were prepared with older embryos of 5-, 8.5-, 14-, and 16-days of incubation, no differentiation of lens and PE cells was observed.
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U2 - 10.1111/j.1432-0436.1982.tb01247.x
DO - 10.1111/j.1432-0436.1982.tb01247.x
M3 - Article
C2 - 7173526
AN - SCOPUS:0020410175
SN - 0301-4681
VL - 22
SP - 179
EP - 184
JO - Differentiation
JF - Differentiation
IS - 1-3
ER -
