Differentiation of phytopathogenic Pseudomonas and Xanthomonas pathovars and strains by PCR analysis for DNA topoisomerase genes

Abu Ashraf Khan, Naruto Furuya, Matsumoto Masaru, Nobuaki Matsuyama

Research output: Contribution to journalArticle

Abstract

Differentiation of pathovars of Pseudomonas syringae and Xanthomonas campestris was conducted by analysis with polymerase chain reaction (PCR) of topoismerase genes. Differences among the pathovars were observed on the migration patterns of the PCR products on agarose gel. Banding patterns of respective strains were pathovar specific with some exceptions. The technique is rapid, simple and reproductive to identify and classify phytopathogenic Pseudomonas syringae and Xanthomonas campestris at pathovar level, and it may be a useful diagnostic tool for these important plant pathogens.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number1
Publication statusPublished - Nov 1 2000

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Xanthomonas campestris
Xanthomonas
DNA topoisomerase
Pseudomonas syringae
Type I DNA Topoisomerase
Pseudomonas
polymerase chain reaction
Polymerase Chain Reaction
Sepharose
Genes
genes
Gels
plant pathogens
agarose
gels
pathovars

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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AU - Masaru, Matsumoto

AU - Matsuyama, Nobuaki

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