Differentiation of soybean-nodulating Bradyrhizobium USDA strains using restriction fragment length polymorphism analysis of 23S-5S rRNA genes

Yuichi Saeki, Tadashi Murata, Takeo Yamakawa, Shoichiro Akao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cluster analysis of the electrophoresis patterns from polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of approximately 3 kbp of the 23S-5S rRNA genes (rDNA) of 21 Bradyrhizobium USDA strains and a Sinorhizobium fredii type strain was conducted to determine the usefulness of these genes for differentiating soybean-nodulating bacteria. The PCR-RFLP analysis of 23S-5S rDNAs separately digested by each of the four restriction enzymes HhaI, HinfI, MboI, and XspI detected 20-26 restriction fragments longer than 100 bp and a total of 13 RFLP patterns in the Bradyrhizobium USDA strains tested, which were distinguishable from the S. fredii type strain. Thus, PCR-RFLP analysis of 23S-5S rDNAs is a simple and useful approach to the grouping of soybean-nodulating isolates, although there were topological differences between the results of PCR-RFLP analysis of these genes and of the 16S-23S rDNA intergenic transcribed spacer region. Depending on the design of the analysis, it is necessary to choose the appropriate target region (i.e. the 16S-23S rDNA ITS region or the 23S-5S rDNA) because of differences in the number of operational taxonomic units in the resolutions of each target beween Bradyrhizobium japonicum and Bradyrhizobium elkanii.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalSoil Science and Plant Nutrition
Volume53
Issue number5
DOIs
Publication statusPublished - Oct 1 2007

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Bradyrhizobium
USDA
polymerase chain reaction
soybean
restriction fragment length polymorphism
polymorphism
ribosomal RNA
soybeans
Sinorhizobium fredii
gene
genes
Bradyrhizobium elkanii
Bradyrhizobium japonicum
cluster analysis
electrokinesis
electrophoresis
enzyme
bacterium
analysis
bacteria

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Agronomy and Crop Science

Cite this

Differentiation of soybean-nodulating Bradyrhizobium USDA strains using restriction fragment length polymorphism analysis of 23S-5S rRNA genes. / Saeki, Yuichi; Murata, Tadashi; Yamakawa, Takeo; Akao, Shoichiro.

In: Soil Science and Plant Nutrition, Vol. 53, No. 5, 01.10.2007, p. 562-567.

Research output: Contribution to journalArticle

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