TY - GEN
T1 - Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane
AU - Benial, A. Milton Franklin
AU - Meenakumari, V.
AU - Ichikawa, Kazuhiro
AU - Yamada, Ken Ichi
AU - Hyodo, Fuminori
AU - Jawahar, A.
AU - Utsumi, Hideo
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.
AB - Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.
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U2 - 10.1063/1.4872551
DO - 10.1063/1.4872551
M3 - Conference contribution
AN - SCOPUS:84903122134
SN - 9780735412255
T3 - AIP Conference Proceedings
SP - 222
EP - 224
BT - Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013
PB - American Institute of Physics Inc.
T2 - 58th DAE Solid State Physics Symposium 2013
Y2 - 17 December 2013 through 21 December 2013
ER -