Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane

A. Milton Franklin Benial, V. Meenakumari, Kazuhiro Ichikawa, Ken Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.

Original languageEnglish
Title of host publicationSolid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013
PublisherAmerican Institute of Physics Inc.
Number of pages3
ISBN (Print)9780735412255
Publication statusPublished - Jan 1 2014
Event58th DAE Solid State Physics Symposium 2013 - Patiala, Punjab, India
Duration: Dec 17 2013Dec 21 2013

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Other58th DAE Solid State Physics Symposium 2013
CityPatiala, Punjab

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)


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