Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study

V. Meenakumari, Hideo Utsumi, Kazuhiro Ichikawa, Ken Ichi Yamada, Fuminori Hyodo, A. Jawahar, A. Milton Franklin Benial

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electron spin resonance (ESR) studies were carried out for permeable 2mM 14 N-labeled deutrated 3 Methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 1mM, 2mM, 3mM, 4mM concentration of 14N-labeled deutrated MC-PROXYL in 400mM concentration of liposomal solution by using a 300 MHz ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported for these samples. The line broadening was observed for the nitroxyl spin probe in the liposomal solution. The line broadening indicates that the high viscous nature of the liposomal solution. The partition parameter and permeability values indicate the maximum diffusion of nitroxyl spin probes in the bilayer lipid membranes at 2 mM concentration of nitroxyl radical. This study illustrates that ESR can be used to differentiate between the intra and extra- membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the spin probe concentration was optimized as 2mM in liposomal solution for ESR phantom studies/imaging, invivo and invitro experiments.

Original languageEnglish
Title of host publicationSolid State Physics
Subtitle of host publicationProceedings of the 59th DAE Solid State Physics Symposium 2014
EditorsR. Chitra, N. K. Sahoo, Dibyendu Bhattacharyya
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413108
DOIs
Publication statusPublished - Jun 24 2015
Event59th DAE Solid State Physics Symposium, SSPS 2014 - Vellore, Tamilnadu, India
Duration: Dec 16 2014Dec 20 2014

Publication series

NameAIP Conference Proceedings
Volume1665
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference59th DAE Solid State Physics Symposium, SSPS 2014
CountryIndia
CityVellore, Tamilnadu
Period12/16/1412/20/14

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

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