Digoxin transport by renal proximal tubule cells is enhanced by adhesive synthetic RGD peptide

Hiroyuki Ijima, S. Kuroda, T. Ono, K. Kawakami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: The dialyzer apparatus has been widely used as an artificial kidney in medical treatment. However, side effects such as amyloidosis have occurred during long-term treatment. Therefore, we focused on developing a hybrid artificial kidney with a filtration and reabsorption apparatus, but it was found that cells spread extensively and it is difficult to maintain a uniform monolayer with a regular cell shape on a collagen-coated substrate. The purpose of this study was to improve cell adhesion, uniform stable monolayer formation and active transport function by immobilization of arginine-glycine-aspartic acid (RGD) on the culture substratum. Materials and Methods: Polycarbonate semipermeable membranes were coated with collagen, fibronectin, laminin and synthetic polypeptide, including RGD (Pronectin F). Cell adhesion and digoxin transport were estimated using a renal proximal tubule cell line that overexpressed the P-glycoprotein gene. Results and Discussion: Under initial and confluent conditions, immobilized cell density in Pronectin F-coated wells was higher than that under other conditions. Transepithelial electrical resistance and digoxin transport activity on Pronectin F-coated membranes were the highest of all conditions. This might have been caused by uniform cell morphology and high cell density.

Original languageEnglish
Pages (from-to)25-33
Number of pages9
JournalInternational Journal of Artificial Organs
Volume30
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

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Proximal Kidney Tubule
Digoxin
Cell adhesion
Collagen
Adhesives
Peptides
Artificial Kidneys
Monolayers
polycarbonate
Cells
Membranes
Cell Adhesion
Arginine
Glycoproteins
Acoustic impedance
Polypeptides
Cell Count
Polycarbonates
Amino acids
Immobilized Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Digoxin transport by renal proximal tubule cells is enhanced by adhesive synthetic RGD peptide. / Ijima, Hiroyuki; Kuroda, S.; Ono, T.; Kawakami, K.

In: International Journal of Artificial Organs, Vol. 30, No. 1, 01.01.2007, p. 25-33.

Research output: Contribution to journalArticle

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