Dihydrostreptomycin goes through the mechano-electric transduction channel in chick cochlear hair cells

Takashi Kimitsuki, Takahiro Wakasaki, Ayako Nawate, Noritaka Komune, Kazutaka Takaiwa, Mitsuru Ohashi, Shizuo Komune

Research output: Contribution to journalArticle

Abstract

Objective: This study evaluated the ability of dihydrostreptomycin (DHSM) to go through the mechano-electric transduction (MET) channels in hair cells under physiological conditions. Materials and Methods: Tall hair cells were isolated from the chick basilar membrane (cochlea). Mechanical stimulation was applied by a glass rod attached to a piezoelectric bimorph, and MET currents were recorded with a whole-cell patch technique. The voltage-dependent block of DHSM to MET channel was estimated by calculating the relative conductances (the ratio of MET current in DHSM saline to DHSM-free saline) at various membrane potentials. Results and Conclusion: At membrane potentials between -100 and +50 mV, DHSM behaves as a voltage-dependent blocker according to a partial block model. At membrane potentials more negative than -100 mV, however, DHSM blocking decreased. This finding differed from the partial block model, but indicated that DHSM escaped through the channel pore into the cytoplasm by acting as a permeant channel blocker due to the large electrical driving force.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalORL
Volume71
Issue number3
DOIs
Publication statusPublished - Jul 1 2009

Fingerprint

Auditory Hair Cells
Dihydrostreptomycin Sulfate
Membrane Potentials
Basilar Membrane
Cochlea
Glass
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Dihydrostreptomycin goes through the mechano-electric transduction channel in chick cochlear hair cells. / Kimitsuki, Takashi; Wakasaki, Takahiro; Nawate, Ayako; Komune, Noritaka; Takaiwa, Kazutaka; Ohashi, Mitsuru; Komune, Shizuo.

In: ORL, Vol. 71, No. 3, 01.07.2009, p. 157-162.

Research output: Contribution to journalArticle

Kimitsuki, Takashi ; Wakasaki, Takahiro ; Nawate, Ayako ; Komune, Noritaka ; Takaiwa, Kazutaka ; Ohashi, Mitsuru ; Komune, Shizuo. / Dihydrostreptomycin goes through the mechano-electric transduction channel in chick cochlear hair cells. In: ORL. 2009 ; Vol. 71, No. 3. pp. 157-162.
@article{c0cf67c6099c43f88c91569cad44ef97,
title = "Dihydrostreptomycin goes through the mechano-electric transduction channel in chick cochlear hair cells",
abstract = "Objective: This study evaluated the ability of dihydrostreptomycin (DHSM) to go through the mechano-electric transduction (MET) channels in hair cells under physiological conditions. Materials and Methods: Tall hair cells were isolated from the chick basilar membrane (cochlea). Mechanical stimulation was applied by a glass rod attached to a piezoelectric bimorph, and MET currents were recorded with a whole-cell patch technique. The voltage-dependent block of DHSM to MET channel was estimated by calculating the relative conductances (the ratio of MET current in DHSM saline to DHSM-free saline) at various membrane potentials. Results and Conclusion: At membrane potentials between -100 and +50 mV, DHSM behaves as a voltage-dependent blocker according to a partial block model. At membrane potentials more negative than -100 mV, however, DHSM blocking decreased. This finding differed from the partial block model, but indicated that DHSM escaped through the channel pore into the cytoplasm by acting as a permeant channel blocker due to the large electrical driving force.",
author = "Takashi Kimitsuki and Takahiro Wakasaki and Ayako Nawate and Noritaka Komune and Kazutaka Takaiwa and Mitsuru Ohashi and Shizuo Komune",
year = "2009",
month = "7",
day = "1",
doi = "10.1159/000219677",
language = "English",
volume = "71",
pages = "157--162",
journal = "ORL",
issn = "0301-1569",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Dihydrostreptomycin goes through the mechano-electric transduction channel in chick cochlear hair cells

AU - Kimitsuki, Takashi

AU - Wakasaki, Takahiro

AU - Nawate, Ayako

AU - Komune, Noritaka

AU - Takaiwa, Kazutaka

AU - Ohashi, Mitsuru

AU - Komune, Shizuo

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Objective: This study evaluated the ability of dihydrostreptomycin (DHSM) to go through the mechano-electric transduction (MET) channels in hair cells under physiological conditions. Materials and Methods: Tall hair cells were isolated from the chick basilar membrane (cochlea). Mechanical stimulation was applied by a glass rod attached to a piezoelectric bimorph, and MET currents were recorded with a whole-cell patch technique. The voltage-dependent block of DHSM to MET channel was estimated by calculating the relative conductances (the ratio of MET current in DHSM saline to DHSM-free saline) at various membrane potentials. Results and Conclusion: At membrane potentials between -100 and +50 mV, DHSM behaves as a voltage-dependent blocker according to a partial block model. At membrane potentials more negative than -100 mV, however, DHSM blocking decreased. This finding differed from the partial block model, but indicated that DHSM escaped through the channel pore into the cytoplasm by acting as a permeant channel blocker due to the large electrical driving force.

AB - Objective: This study evaluated the ability of dihydrostreptomycin (DHSM) to go through the mechano-electric transduction (MET) channels in hair cells under physiological conditions. Materials and Methods: Tall hair cells were isolated from the chick basilar membrane (cochlea). Mechanical stimulation was applied by a glass rod attached to a piezoelectric bimorph, and MET currents were recorded with a whole-cell patch technique. The voltage-dependent block of DHSM to MET channel was estimated by calculating the relative conductances (the ratio of MET current in DHSM saline to DHSM-free saline) at various membrane potentials. Results and Conclusion: At membrane potentials between -100 and +50 mV, DHSM behaves as a voltage-dependent blocker according to a partial block model. At membrane potentials more negative than -100 mV, however, DHSM blocking decreased. This finding differed from the partial block model, but indicated that DHSM escaped through the channel pore into the cytoplasm by acting as a permeant channel blocker due to the large electrical driving force.

UR - http://www.scopus.com/inward/record.url?scp=66049112912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66049112912&partnerID=8YFLogxK

U2 - 10.1159/000219677

DO - 10.1159/000219677

M3 - Article

C2 - 19468245

AN - SCOPUS:66049112912

VL - 71

SP - 157

EP - 162

JO - ORL

JF - ORL

SN - 0301-1569

IS - 3

ER -