Dipyridamole potentiates adriamycin cytotoxicity by a mechanism other than inhibiting nucleoside uptake

H. Kusumoto; Y. Maehara; H. Anai; R. Kumashiro; K. Sugimachi

Dipyridamole potentiates adriamycin cytotoxicity by a mechanism other than inhibiting nucleoside uptake

H. Kusumoto, Y. Maehara, H. Anai, R. Kumashiro, K. Sugimachi

Surgery

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Nitrobenzylthioinosine (NBTI) and dipyridamole (DP) are competitive inhibitors of cellular nucleoside uptake. Although combined treatment of HeLa cells with adriamycin (ADM) and DP enhanced ADM cytotoxicity in cell growth and clonogenic assays, the combination of ADM and noncytostatic levels (less than 1 μM) of NBTI did not change the cytotoxic potential of ADM in vitro. DP enhanced the inhibition of clonogenicity by ADM, even in nucleoside-enriched mediums. These results suggest that the synergy between ADM and DP was hardly due to the inhibition of nucleoside uptake by DP, but was due to the enhancement of intracellular ADM accumulation by DP.

Original language	English
Pages (from-to)	1539-1542
Number of pages	4
Journal	Anticancer research
Volume	11
Issue number	4
Publication status	Published - 1991

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{c5745516115448aea56353c6a7601e34,

title = "Dipyridamole potentiates adriamycin cytotoxicity by a mechanism other than inhibiting nucleoside uptake",

abstract = "Nitrobenzylthioinosine (NBTI) and dipyridamole (DP) are competitive inhibitors of cellular nucleoside uptake. Although combined treatment of HeLa cells with adriamycin (ADM) and DP enhanced ADM cytotoxicity in cell growth and clonogenic assays, the combination of ADM and noncytostatic levels (less than 1 μM) of NBTI did not change the cytotoxic potential of ADM in vitro. DP enhanced the inhibition of clonogenicity by ADM, even in nucleoside-enriched mediums. These results suggest that the synergy between ADM and DP was hardly due to the inhibition of nucleoside uptake by DP, but was due to the enhancement of intracellular ADM accumulation by DP.",

author = "H. Kusumoto and Y. Maehara and H. Anai and R. Kumashiro and K. Sugimachi",

year = "1991",

language = "English",

volume = "11",

pages = "1539--1542",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

}

TY - JOUR

T1 - Dipyridamole potentiates adriamycin cytotoxicity by a mechanism other than inhibiting nucleoside uptake

AU - Kusumoto, H.

AU - Maehara, Y.

AU - Anai, H.

AU - Kumashiro, R.

AU - Sugimachi, K.

PY - 1991

Y1 - 1991

N2 - Nitrobenzylthioinosine (NBTI) and dipyridamole (DP) are competitive inhibitors of cellular nucleoside uptake. Although combined treatment of HeLa cells with adriamycin (ADM) and DP enhanced ADM cytotoxicity in cell growth and clonogenic assays, the combination of ADM and noncytostatic levels (less than 1 μM) of NBTI did not change the cytotoxic potential of ADM in vitro. DP enhanced the inhibition of clonogenicity by ADM, even in nucleoside-enriched mediums. These results suggest that the synergy between ADM and DP was hardly due to the inhibition of nucleoside uptake by DP, but was due to the enhancement of intracellular ADM accumulation by DP.

AB - Nitrobenzylthioinosine (NBTI) and dipyridamole (DP) are competitive inhibitors of cellular nucleoside uptake. Although combined treatment of HeLa cells with adriamycin (ADM) and DP enhanced ADM cytotoxicity in cell growth and clonogenic assays, the combination of ADM and noncytostatic levels (less than 1 μM) of NBTI did not change the cytotoxic potential of ADM in vitro. DP enhanced the inhibition of clonogenicity by ADM, even in nucleoside-enriched mediums. These results suggest that the synergy between ADM and DP was hardly due to the inhibition of nucleoside uptake by DP, but was due to the enhancement of intracellular ADM accumulation by DP.

UR - http://www.scopus.com/inward/record.url?scp=0025837230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025837230&partnerID=8YFLogxK

M3 - Article

C2 - 1746911

AN - SCOPUS:0025837230

VL - 11

SP - 1539

EP - 1542

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4

ER -

Dipyridamole potentiates adriamycin cytotoxicity by a mechanism other than inhibiting nucleoside uptake

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Other files and links

Fingerprint

Cite this