Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes

Shimpei Aikawa, Ancy Joseph, Ryosuke Yamada, Yoshihiro Izumi, Takahiro Yamagishi, Fumio Matsuda, Hiroshi Kawai, Jo Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as feedstocks for next-generation biofuels. To date, however, there have been no reports on efficient bioethanol production from cyanobacterial glycogen by yeast fermentation. Additionally, multiple pretreatment and enzymatic hydrolysis steps of polysaccharides are required for conventional ethanol production from agricultural crops and microalgae. Here, we investigate direct ethanol production from Arthrospira (Spirulina) platensis, a fast-growing halophilic cyanobacterium that accumulates large amounts of glycogen, using lysozyme and a recombinant amylase-expressing yeast strain to eliminate the need for biomass pretreatment and amylase hydrolysis. In the direct conversion process from A. platensis to ethanol, 6.5 g L-1 (ethanol productivity of 1.08 g per L per day) of ethanol was produced. The total ethanol yield based on glycogen consumption was 86% of theoretical yield, which to our knowledge, is the highest yield of bioethanol from an oxygenic photosynthetic microorganism. The present findings indicate that A. platensis is a remarkable carbohydrate feedstock in the form of glycogen, which is a promising material for the production of bioethanol and various other commercially valuable chemicals.

Original languageEnglish
Pages (from-to)1844-1849
Number of pages6
JournalEnergy and Environmental Science
Volume6
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Fingerprint

Spirulina
Enzymatic hydrolysis
ethanol
hydrolysis
Ethanol
Glycogen
Bioethanol
Amylases
Microorganisms
Yeast
Feedstocks
yeast
cyanobacterium
microorganism
Biofuels
Polysaccharides
Carbohydrates
Muramidase
polysaccharide
biofuel

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Environmental Chemistry
  • Pollution
  • Nuclear Energy and Engineering

Cite this

Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes. / Aikawa, Shimpei; Joseph, Ancy; Yamada, Ryosuke; Izumi, Yoshihiro; Yamagishi, Takahiro; Matsuda, Fumio; Kawai, Hiroshi; Chang, Jo Shu; Hasunuma, Tomohisa; Kondo, Akihiko.

In: Energy and Environmental Science, Vol. 6, No. 6, 06.2013, p. 1844-1849.

Research output: Contribution to journalArticle

Aikawa, S, Joseph, A, Yamada, R, Izumi, Y, Yamagishi, T, Matsuda, F, Kawai, H, Chang, JS, Hasunuma, T & Kondo, A 2013, 'Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes', Energy and Environmental Science, vol. 6, no. 6, pp. 1844-1849. https://doi.org/10.1039/c3ee40305j
Aikawa, Shimpei ; Joseph, Ancy ; Yamada, Ryosuke ; Izumi, Yoshihiro ; Yamagishi, Takahiro ; Matsuda, Fumio ; Kawai, Hiroshi ; Chang, Jo Shu ; Hasunuma, Tomohisa ; Kondo, Akihiko. / Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes. In: Energy and Environmental Science. 2013 ; Vol. 6, No. 6. pp. 1844-1849.
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